单免疫球蛋白-白介素1受体蛋白在慢性乙肝患者中的表达水平变化及临床意义研究  

Changes in the Expression Level and Clinical Significance of Monoimmunoglobulin-interleukin 1 Receptor Protein in Patients with Chronic Hepatitis B

作　　者：吴刚[1] 叶晓玲[1] 张宇 石磬 WU Gang;YE Xiao-ling;ZHANG Yu;SHI Qing(Liver Disease Department,Jiujiang First People's Hospital,Jiujiang 332000,Jiangxi Province,China)

机构地区：[1]九江市第一人民医院肝病科,江西九江332000

出　　处：《罕少疾病杂志》2023年第12期72-74,共3页Journal of Rare and Uncommon Diseases

基　　金：江西省卫健委立项课题(202211889)。

摘　　要：目的分析慢性乙型肝炎(CHB)患者中单免疫球蛋白-白介素1受体蛋白(SIGIRR)的表达水平改变和临床意义。方法选取2022.01-2022.12本院收治的CHB患者60例设为观察组,另选取同时间段内本院健康体检人群50例设为对照组,两组测定外周血单个核细胞(PBMC)中SIGIRR mRNA表达、SIGIRR+细胞频率及CD3+SIGIRR+T淋巴细胞比率;外周血Th1和Treg细胞频率;血清干扰素-γ(IFN-γ)、白细胞介素-10(IL-10)、白细胞介素-6(IL-6)、白细胞介素-18(IL-18)及肿瘤坏死因子-α(TNF-α)浓度;PBMC中Toll样受体4(TLR4)的mRNA水平;NK细胞表面NK细胞受体2A抗体(NKG2A)表达,比较两组各项指标差异,并分析SIGIRR和各项指标的相关性。结果观察组PBMC中SIGIRR mRNA表达、SIGIRR+细胞频率及CD3+SIGIRR+T淋巴细胞比率低于对照组(P<0.05),TLR4的mRNA高于对照组(P<0.05)。观察组外周血Th1和Treg细胞频率高于对照组(P<0.05)。观察组血清IFN-γ、IL-10及IL-18浓度低于对照组(P<0.05),IL-6及TNF-α高于对照组(P<0.05)。观察组NK细胞表面NKG2A的表达水平高于对照组(P<0.05)。SIGIRR和TLR4的mRNA、Th1细胞频率、Treg细胞频率、IL-6、TNF-α、NK细胞表面NKG2A表达水平之间均为负相关关系(P<0.05),和IFN-γ、IL-10、IL-18水平之间均为正相关关系(P<0.05)。结论CHB患者SIGIRR表达水平异常,且和免疫功能紊乱有关。CHB患者的SIGIRR低表达能直接或者间接活化TLR4,上调NK细胞表面NKG2A和IL-6及TNF-α等细胞因子表达,下调IFN-γ、IL-10及IL-18等细胞因子表达,进而导致免疫调节功能紊乱。Objective To analyze the altered expression level and clinical significance of monoimmunoglobulin-interleukin 1 receptor protein(SIGIRR)in patients with chronic hepatitis B(CHB).Methods 60 CHB patients admitted in 2022.01-2022.12 hospital were selected as the observation group,In addition,50 patients with health examination in the same period were selected as the control group,Two groups determined SIGIRR mRNA expression,SIGIRR+cell frequency,and CD3+SIGIRR+T lymphocyte ratio in peripheral blood mononuclear cells(PBMC);Frequency of Th 1 and Treg cells in peripheral blood;Concentrations of serum interferon-γ(IFN-γ),interleukin-10(IL-10),interleukin-6(IL-6),interleukin-18(IL-18),and tumor necrosis factor-α(TNF-α);The mRNA level of Toll-like receptor 4(TLR 4)in PBMC;NK cell surface NK cell receptor 2A antibody(NKG2A)expression,Comparing the differences between the various indicators of the two groups,And analyzed the correlation of SIGIRR and various indicators.Results SIGIRR mRNA expression,SIGIRR+cell frequency and CD3+SIGIRR+T lymphocyte ratio in PBMC were lower(P<0.05)and mRNA in TLR 4 was higher than the control group(P<0.05).The frequency of peripheral Th 1 and Treg cells in the observation group was higher than that in the control group(P<0.05).Serum concentrations of IFN-γ,IL-10 and IL-18 in the observation group were lower than the control group(P<0.05),and IL-6 and TNF-αwere higher than the control group(P<0.05).The expression level of NKG 2 A in the NK group was higher than that of the control group(P<0.05).A negative correlation between mRNA,Th 1,IL-6,TNF-α,NKG 2 A and TLR 4(P<0.05)and a positive correlation between IFN-γ,IL-10,and IL-18 levels(P<0.05).Conclusion CHB patients have abnormal SIGIRR expression levels and a disordered immune function.The low expression of SIGIRR in CHB patients can directly or indirectly activate TLR 4,upregulate the expression of cytokines such as NKG2A and IL-6 and TNF-αon the surface of NK cells,and downregulate the expression of cytokines such as IFN-γ,IL-IL-10

关 键 词：慢性乙型肝炎 单免疫球蛋白-白介素1受体蛋白 免疫功能 相关性 

分 类 号：R512.62[医药卫生—内科学]