水稻粒型与垩白性状相关分析和QTL定位  

Correlation analysis between rice grain shape and chalkiness traitsand QTL mapping

作　　者：崔磊 韩雷锋 王晨 林翠香 甘泉 倪大虎[2] 吴德祥[1] 宋丰顺[2] CUI Lei;HAN Leifeng;WANG Cheng;LIN Cuixiang;GAN Quan;NI Dahu;WU Dexiang;SONG Fengshun(College of Agronomy,Anhui Agricultural University,Hefei 230036,China;Rice Research Institute,Anhui Academy of Agricultural Science,Hefei 230031,China)

机构地区：[1]安徽农业大学农学院,合肥230036 [2]安徽省农业科学院水稻研究所,合肥230031

出　　处：《生物学杂志》2023年第6期60-64,共5页Journal of Biology

基　　金：安徽省遗传育种重点实验室开放基金项目(SDKF-2020-01);安徽省自然科学基金项目(2008085MC98);安徽省科技重大专项项目(101164076024);安徽省水稻良种联合攻关项目。

摘　　要：以籼型水稻RH003与华占构建的106个重组自交系后代作为试验材料,对海南陵水和安徽合肥两季稻米的粒长、粒宽、长宽比、垩白粒率和垩白度5个外观品质性状进行相关性分析和QTL定位,研究发现垩白与长宽比相关性最高,呈极显著负相关,其次为粒宽,呈显著正相关,粒长与垩白相关性较小,仅合肥试验点的粒长和垩白度达到负相关显著水平,其他均未达到显著水平;QTL定位共检测到17个QTL位点,两季重复检测到的QTL位点有6个(qGL2a、qGL2b、qGW2b、qGLW2、qCR4和qCD4),且LOD值和表型贡献率较高。其中,粒型QTL 4个,均位于第二染色体,垩白度和垩白粒率QTL各1个,均位于4号染色体且位置相近。研究可为外观品种性状的基因发掘和分子育种提供参考。Using 106 recombinant inbred line progeny constructed by Indica rice RH003 and Huachan as experimental materials,correlation analysis and QTL mapping were conducted for five appearance and quality traits including grain length,grain width,length-to-width ratio,chalkiness grain rate and chalkiness degree of rice planted in the different places from Lingshui,Hainan province and Hefei,Anhui province.The results found that the correlation between chalkiness and length-width ratio was the highest and showed an extremely significant negative correlation.The grain width showed a significant positive correlation.Grain length had little correlation with chalkiness.The season grain length and chalkiness in Hefei reached a significant level of negative correlation,while the others did not reach a significant level.A total of 17 QTL were detected by QTL mapping,and 6 QTL loci(qGL2a,qGL2b,qGW2b,qGLW2,qCR4 and qCD4)were repeatedly detected in two seasons,and the LOD value and phenotypic contribution rate were higher.There were 4 QTLS for grain type,all locating on the second chromosome,and 1 QTL for chalkiness and chalkiness rate locating on the fourth chromosome with similar locations.This study could provide reference for gene discovery and molecular breeding of appearance varieties.

关 键 词：水稻 垩白 粒型 QTL 相关性 

分 类 号：S511[农业科学—作物学]