炭药提取液对小鼠皮肤伤口愈合过程中胶原组成的影响  

Effects of charcoal medicine extract on collagen compositionin skin wound healing of mice

作　　者：王俊杰 高建萍[2] 张扬[2] 张贵锋[2] 马莉 WANG Junjie;GAO Jianping;ZHANG Yang;ZHANG Guifeng;MA Li(School of Pharmacy,Chengdu University of Traditional Chinese Medicine,Chengdu 611137,China;State Key Laboratory of Biochemical Engineering,Institute of Process Engineering,Beijing 100190,China;School of Traditional Chinese Medicine,Capital Medical University,Beijing 100069,China)

机构地区：[1]成都中医药大学药学院,成都611137 [2]中国科学院过程工程研究所生化工程国家重点实验室,北京100190 [3]首都医科大学中医药学院,北京100069

出　　处：《生物学杂志》2023年第6期88-93,共6页Journal of Biology

基　　金：国家重点研发计划项目(No.2021YFC2400804)。

摘　　要：研究炭药提取液(液体敷料1与液体敷料2)对小鼠皮肤伤口愈合过程中Ⅰ、Ⅲ胶原蛋白含量变化的影响。以小鼠全皮层创伤为模型,将液体敷料1、液体敷料2涂抹于小鼠背部伤口,给药3、6、9、13、17和21 d后,通过液质联用法测定小鼠创面组织中Ⅰ、Ⅲ型胶原含量。结果表明:液体敷料1与液体敷料2能明显提高创面愈合率并加速结痂掉落,并在第9、13天明显增加总胶原的含量(P<0.01);第3、9天,液体敷料1与液体敷料2组的Ⅰ、Ⅲ型胶原含量均大于空白组(P<0.05);且在第17、21天,两组胶原Ⅰ/Ⅲ的比例均大于空白组(P<0.01)。研究为探索炭药提取液促进伤口愈合的机理提供基础。The study determined the effect of charcoal medicine extract(liquid dressing 1 and liquid dressing 2)on the changes of typeⅠandⅢcollagen content in the healing process of skin wounds in mice.A model of full-thickness wound in mice was established.Liquid dressing 1 and liquid dressing 2 were applied daily on the back of mice.After 3,6,9,13,17 and 21 days of administration,the content of collagenⅠandⅢin wound tissues of mice was determined by HPLC-MS.Results showed that liquid dressing 1 and liquid dressing 2 can obviously improve the wound healing rate and accelerate the scab shedding.Compared with the control group,liquid dressing 1 and liquid dressing 2 could obviously promote the synthesis of total collagen at 9 d and 13 d(P<0.01).The content of typeⅠand typeⅢcollagen in liquid dressing 1 and liquid dressing 2 were greater than that of control group at 3 d and 9 d(P<0.05).The ratio of collagenⅠ/Ⅲin liquid dressing 1 and liquid dressing 2 were greater than that of control group at 17 d and 21 d(P<0.01).This study provided a theoretical basis for the treatment of wound healing with charcoal medicine extract.

关 键 词：炭药提取液 创面愈合 Ⅰ型胶原 Ⅲ型胶原 成纤维细胞 

分 类 号：Q25[生物学—细胞生物学]