利血平对脊髓小脑共济失调3型(SCA3)细胞模型的影响  

Effect of reserpine on spinocerebellar ataxia type 3(SCA3)cell model

作　　者：王子见 马芝薇 何树妮 任媛媛 王琳琳[1] 石玉琪 刘婷 李子恒 何凤琴[1] WANG Zi-jian;MA Zhi-wei;HE Shu-ni;REN Yuan-yuan;WANG Lin-lin;SHI Yu-qi;LIU Ting;LI Zi-heng;HE Feng-qin(Key Laboratory of Natural Product Development and Anticancer Innovative Drug Research in Qinling,Xi’an University,Xi’an 710065,China)

机构地区：[1]西安文理学院西安市秦岭天然产物开发与创新药物研究重点实验室,陕西西安710065

出　　处：《现代药物与临床》2023年第11期2672-2678,共7页Drugs & Clinic

基　　金：国家自然科学基金资助项目(82202067,32270530);陕西省自然科学基础研究计划资助项目(2020JM-620);西安市科技计划创新基金“文理专项”项目(2020KJWL04);西安文理学院博士启动金(06005026);西安文理学院院长基金(YZJJ202104);国家级大学生创新创业项目(S202011080012,S202311080020);省级大学生创新创业项目(S202011080058,S202111080043,S202111080031)

摘　　要：目的 阐明利血平对脊髓小脑共济失调3型(SCA3)细胞模型的潜在作用。方法 通过核质分离、免疫荧光、Western blotting、微孔过滤和细胞活性等实验探索利血平对SCA3发病过程中ataxin-3核质转运、ataxin-3可溶性蛋白水平、蛋白包涵体、细胞活性等一系列病理变化的影响。结果 利血平对ataxin-3核质转运和可溶性蛋白水平没有显著性影响,但是增加了ataxin-3形成的包涵体数量,并且增加了细胞毒性。结论 利血平通过增加包涵体数量导致SCA3细胞活性减弱,为老药新用发现提供了理论依据。Objective To clarify the potential effect of reserpine on spinocerebellar ataxia type 3(SCA3)cell model.Methods The effects of reserpine on ataxin-3 nuclear and cytoplasmic transport,ataxin-3 soluble protein level,protein inclusion body and cell viability during the pathogenesis of SCA3 were investigated by nucleocytoplasmic separation,immunofluorescence,Western blotting,filter trap and CCK-8 methods.Results Reserpine had no significant effect on the nucleocytoplasmic transport and soluble protein level of ataxin-3,but increased the number of inclusion bodies formed by ataxin-3 and increased cytotoxicity.Conclusion Reserpine decreases the SCA3 cell viability by increasing the number of inclusion bodies,which provides a theoretical basis for the new discovery of old drugs.

关 键 词：利血平 脊髓小脑共济失调3型 ATAXIN-3 蛋白包涵体 核质转运 可溶性蛋白 

分 类 号：R966[医药卫生—药理学]