生物素修饰的Fe_(3)O_(4)纳米粒子作为抗癌药物载体的应用研究  

Application of Biotin-modified Fe_(3)O_(4) Nanaoparticles as Anti-cancer Drug Carriers

作　　者：弓韬[1,2] 侯雪怡 王晓宇 文朝朝[2] 黄昱 张雅静 郭睿 梁文婷[2] GONG Tao;HOU Xueyi;WANG Xiaoyu;WEN Chaochao;HUANG Yu;ZHANG Yajing;GUO Rui;LIANG Wenting(Department of Biochemistry and Molecular Biology,Shanxi Medical University,Taiyuan 030001,China;Institute of Environmental Science,Shanxi University,Taiyuan 030006,China)

机构地区：[1]山西医科大学生物化学与分子生物学教研室,山西太原030001 [2]山西大学环境科学研究所,山西太原030006

出　　处：《山西大学学报（自然科学版）》2023年第6期1393-1401,共9页Journal of Shanxi University(Natural Science Edition)

基　　金：国家自然科学基金(21976113);山西省自然科学基金(202103021224240,20210302123322)。

摘　　要：本文将生物素(Biotin)修饰于Fe_(3)O_(4)磁性纳米粒子表面制备了BIO-MNPs纳米材料。盐酸阿霉素(DOX)可以通过与生物素之间的氢键作用和自聚集作用负载于BIO-MNPs表面,实验条件下的最大负载量可达823.6 mg/g,且BIO-MNPs@DOX对DOX的释放在弱酸性环境下更优。体外溶血实验以及细胞毒性实验证明BIO-MNPs具有良好的血液相容性和较低的生物毒性;体外细胞摄取实验证明BIO-MNPs@DOX对肝癌细胞和人乳腺癌细胞具有较好的靶向性能,且具有良好的抑制效果。以上结果表明BIO-MNPs可作为药物载体负载抗癌药物DOX,且BIOMNPs@DOX在癌细胞的靶向抑制方面具有一定的应用价值。In this paper,biotin-modified Fe_(3)O_(4) magnetic nanoparticles(BIO-MNPs)were prepared by the condensation of biotin and Fe_(3)O_(4) nanoparticles.The BIO-MNPs were then loaded with the broad-spectrum anti-cancer drug doxorubicin(DOX)as a therapeutic unit.DOX could effectively load on the surface of BIO-MNPs via biotin regulatory hydrogen-bonding interaction and the p-p stacking-induced self-assembly of drug molecules.The loading capacity of DOX reached up to 823.6 mg/g.In addition,the loaded DOX can be induced to be released by the acidic microenvironment of cancer cells.The vitro hemolysis test and cytotoxicity test proved that BIO-MNPs had good biocompatibility and low biological toxicity.The cell uptake experiment in vitro demonstrated that BIOMNPs@DOX had good targeting performance for liver cancer cells and breast cancer cells,and had efficient inhibition effect on them.These results demonstrated that the BIO-MNPs can be used as a drug carrier to load the anticancer drug DOX and have potential practical application values for the targeted inhibition of cancer cells.

关 键 词：生物素 Fe_(3)O_(4)磁性纳米粒子 盐酸阿霉素 癌症 

分 类 号：O647[理学—物理化学]