丁香苷抑制大鼠椎间盘退变  

Syringin inhibits intervertebral disc degeneration in rats

作　　者：张云鑫 张存鑫 王倩[2] 徐新亮[3] 吕超亮[2] 倪勇[2] Zhang Yunxin;Zhang Cunxin;Wang Qian;Xu Xinliang;Lyu Chaoliang;Ni Yong(School of Clinical Medicine,Jining Medical University,Jining 272067,Shandong Province,China;Department of Spine Surgery,Jining No.1 People’s Hospital,Jining 272000,Shandong Province,China;Department of Pain,Jining No.1 People’s Hospital,Jining 272000,Shandong Province,China)

机构地区：[1]济宁医学院临床医学院,山东省济宁市272067 [2]济宁市第一人民医院脊柱外科,山东省济宁市272000 [3]济宁市第一人民医院疼痛科,山东省济宁市272000

出　　处：《中国组织工程研究》2024年第32期5104-5109,共6页Chinese Journal of Tissue Engineering Research

基　　金：山东省医药卫生科技发展计划项目(202204070552),项目负责人:王倩;山东省医药卫生科技发展计划项目(2021M079),项目负责人:徐新亮;济宁市重点研发计划项目(2021YXNS050),项目负责人:王倩;济宁市重点研发计划项目(2020JKNS008),项目负责人:张存鑫。

摘　　要：背景:椎间盘退变是由于椎间盘内部髓核和纤维环组织发生损伤和退化导致的椎间盘结构和功能发生变化,目前尚无有效的治疗药物。目的:探讨丁香苷抑制大鼠椎间盘退变的作用。方法:取10只雄性SD大鼠,将每只大鼠的尾椎Co_(4)/Co_(5)椎间盘设为模型组、Co_(5)/Co_(6)椎间盘设为丁香苷组、Co_(6)/Co_(7)椎间盘设为对照组,对照组不进行任何处理,模型组、丁香苷组采用微型穿刺针进行纤维环全层穿刺建立椎间盘退变模型,造模后即刻,模型组、丁香苷组椎间盘分别注射2.5μL的生理盐水、丁香苷溶液(5μmol/L)。注射4周后取材,采用苏木精-伊红和番红O-固绿染色观察大鼠椎间盘退变程度,免疫组化染色分析大鼠椎间盘组织内Ⅱ型胶原、聚集蛋白聚糖及基质金属蛋白酶3,13的表达。结果与结论:①苏木精-伊红染色显示,模型组椎间盘高度降低,软骨终板变薄且有裂隙出现,纤维环结构紊乱且出现裂隙,髓核消失;丁香苷组椎间盘高度正常或略低于对照组,软骨终板退变程度较模型组轻,纤维环排列较模型组相对规整且无裂隙,髓核部分皱缩。②番红O-固绿染色显示,模型组椎间盘软骨终板出现缺损且软骨钙化层变薄,出现明显退变;丁香苷组椎间盘软骨终板结构形态有一定程度恢复。③免疫组化染色显示,与对照组比较,模型组椎间盘软骨组织内Ⅱ型胶原、聚集蛋白聚糖的表达降低(P<0.0001),基质金属蛋白酶3,13的表达升高(P<0.0001);与模型组比较,丁香苷组椎间盘软骨组织内Ⅱ型胶原、聚集蛋白聚糖的表达升高(P<0.001,P<0.0001),基质金属蛋白酶3,13的表达降低(P<0.001,P<0.0001)。④结果表明,丁香苷可通过抑制基质金属蛋白酶3,13的表达、提高Ⅱ型胶原和聚集蛋白聚糖的表达来改善椎间盘的结构和功能,预防和减缓椎间盘退变过程。BACKGROUND:Intervertebral disc degeneration is caused by damage and degeneration of the nucleus pulposus and annulus fibrosus tissues inside the intervertebral disc,resulting in structural and functional changes of the intervertebral disc.However,there is yet no effective drug treatment for intervertebral disc degeneration.OBJECTIVE:To investigate the inhibitory effect of syringin on intervertebral disc degeneration.METHODS:A total of 10 male Sprague-Dawley rats were selected,and the coccygeal intervertebral disc(Co_(4)/Co_(5))of each rat was set as model group,Co_(5)/Co_(6)intervertebral disc as syringin group,and Co_(6)/Co_(7)intervertebral disc as control group.The control group did not receive any treatment.In the model group and syringin group,a miniature puncture needle was used to puncture the annulus fibrosus to establish an intervertebral disc degeneration model.Immediately after modeling,2.5μL of normal saline and syringin solution(5μmol/L)were given in the model and syringin groups,respectively.Four weeks after injection,the samples were taken.The degree of intervertebral disc degeneration in rats was observed by hematoxylin-eosin and safranine O-fast green staining.The expressions of type II collagen,aggrecan and matrix metalloproteinases 3 and 13 in intervertebral disc tissue were analyzed by immunohistochemical staining.RESULTS AND CONCLUSION:Hematoxylin-eosin staining showed that in the model group,the height of intervertebral disc decreased,the cartilage endplate became thinner and cracked,the fibrous ring structure was disordered and cracked,and the nucleus pulposus disappeared;in the syringin group,the height of intervertebral disc was normal or slightly lower than that in the control group,the degree of cartilage endplate degeneration was lighter than that in the model group,the fiber circle permutation was relatively regular with no cracks,and the nucleus pulposus was partially shrunk.Safranine O-fast green staining showed that in the model group,the cartilage endplate of the intervertebral

关 键 词：丁香苷 椎间盘退变 腰痛 针刺法 髓核细胞 中药单体 基质金属蛋白酶 

分 类 号：R459.9[医药卫生—治疗学] R319[医药卫生—临床医学] R681.5