IAA作为下游信号参与褪黑素对低温下黄瓜幼苗过氧化伤害的缓解效应  

Auxin acted as a downstream signal participated in the alleviate effect of melatonin on peroxidation damages of cucumber seedlings under chilling stress

作　　者：李俊奇 范玲晓 苑新茹 吕艳辉 艾希珍[1,2,3] 毕焕改 LI Junqi;FAN Lingxiao;YUAN Xinru;LüYanhui;AI Xizhen;BI Huangai(College of Horticulture Science and Engineering,Shandong Agricultural University,Taian,Shandong 271018,China;Key Laboratory of Horticultural Crop Biology and Germplasm Innovation in Huanghuai Region,Ministry of Agriculture and Rural Affairs,Taian,Shandong 271018,China;Collaborative Innovation Center of Shandong Province with High Quality and Efficient Production of Fruit and Vegetable,Taian,Shandong 271018,China)

机构地区：[1]山东农业大学园艺科学与工程学院,山东泰安271018 [2]农业农村部黄淮地区园艺作物生物学与种质创制重点开放实验室,山东泰安271018 [3]山东果蔬优质高效生产协同创新中心,山东泰安271018

出　　处：《植物生理学报》2023年第11期2095-2106,共12页Plant Physiology Journal

基　　金：国家重点研发计划(2018YFD1000800和2019YFD1000300);山东省现代农业产业技术体系建设专项资金(SDAIT-05-10)。

摘　　要：为研究褪黑素(MT)与吲哚乙酸(IAA)在诱导黄瓜耐冷性中存在的关系,以‘津优35号’黄瓜为试材,分别用MT、IAA、氯苯丙氨酸(p-CPA,MT生物合成抑制剂)、N-1-萘基酞氨酸(NPA,IAA极性运输抑制剂)等试剂喷施幼苗叶片。分析常温下MT与IAA的互作效应,以及二者对低温(8℃/5℃)胁迫下黄瓜幼苗抗氧化系统的影响。结果表明,常温下外施MT可显著诱导IAA的积累,而外施IAA对MT含量无影响;喷施MT和IAA均显著缓解低温胁迫对黄瓜幼苗的过氧化伤害;喷施NPA导致MT对低温下黄瓜幼苗过氧化伤害的缓解效果显著减弱,而p-CPA却对IAA诱导的黄瓜耐冷性无影响。综上说明,IAA作为下游信号参与MT对低温下黄瓜幼苗氧化胁迫的调控。In order to study the relationship between melatonin(MT)and indole-3-acetic acid(IAA)in inducing cold tolerance of cucumber,the cultivar‘Jinyou 35'was used as test material,and the leaves of seedlings were sprayed with MT,IAA,chlorophenylalanine(p-CPA,MT biosynthesis inhibitor)and N-1-naphthyl phthalein(NPA,IAA polar transport inhibitor)respectively.The interaction between MT and IAA at room temperature and their effects on antioxidant system of cucumber seedlings under low temperature(8℃/5℃)stress were analyzed.It was found that external application of MT could significantly induce the accumulation of IAA at room temperature,but external application of IAA had no effect on MT content.The application of MT and IAA can significantly alleviate the peroxide damage of cucumber seedlings under low temperature stress.The presence of NPA significantly reduced the alleviation effect of MT on peroxidation damage of cucumber seedlings under low temperature conditions,but IAA-induced cold tolerance was not affected by p-CPA.Therefore,we speculate that IAA,as a downstream signal of MT,was involved in regulating the peroxidation damage of cucumber seedlings under chilling stress.

关 键 词：黄瓜 低温胁迫 褪黑素 生长素 活性氧 抗氧化系统 

分 类 号：S642.2[农业科学—蔬菜学]