酵母菌偏振衍射特征的定量仿真与实验研究  

Quantitative Simulation and Experimental Study of Polarized Diffraction Characteristics of Yeast Cells

作　　者：王文进 张玉霞 撒昱[4] 闵力 田芃 Wang Wenjin;Zhang Yuxia;Sa Yu;Min Li;Tian Peng(School of Physics&Electronic Science,Hunan Institute of Science and Technology,Yueyang 414006,Hunan,China;Key Laboratory of Hunan Province on Information Photonics and Free-Space Optical Communications,Hunan Institute of Science and Technology,Yueyang 414006,Hunan,China;School of Chemistry and Chemical Engineering,Hunan Institute of Science and Technology,Yueyang 414006,Hunan,China;School of Precision Instrument and Opto-Electronics Engineering,Tianjin University,Tianjin 300072,China)

机构地区：[1]湖南理工学院物理与电子科学学院,湖南岳阳414006 [2]湖南理工学院信息光子学与空间光通信湖南省重点实验室,湖南岳阳414006 [3]湖南理工学院化学化工学院,湖南岳阳414006 [4]天津大学精密仪器与光电子工程学院,天津300072

出　　处：《中国激光》2023年第21期123-133,共11页Chinese Journal of Lasers

基　　金：湖南省教育厅重点研究项目(21A0404);湖南省科技计划(2019TP1014)。

摘　　要：偏振光衍射成像技术可以提供丰富的与细胞结构及组成成分相关的信息,在生物细胞无损检测领域具有重要的研究和应用价值。本文对酵母菌结构特征参数与其偏振衍射特征之间的定量关系进行了深入分析,分析结果表明:酵母菌单体短轴尺寸与偏振衍射条纹空间频率之间存在定量关系,基于偏振衍射实验测量图像可以实现酵母菌单体短轴尺寸分布的快速统计,并且酵母菌单体长短轴之比与退偏系数之间成高斯线性相关(R^(2)=0.9986)。此外,结合偏振衍射图像纹理特征与支持向量机聚类模型可以实现酵母菌发芽率的快速统计,准确率为98.1%。利用多元线性回归模型可以预测出酵母菌母体和芽体的短轴比,模型调整后的决定系数为0.86。本研究结果可为基于偏振衍射特征的细胞结构参数反演研究提供借鉴。Objective Polarized diffraction images(p-DIs)can provide a wealth of information about the morphologies of scatterers,making them a valuable tool for use in a variety of applications,including the characterization of biological cells and tissues.However,most studies on biological cells have chiefly relied on qualitative analysis,which is achieved through the analysis of patterns of p-DIs for cell clustering.Although qualitative analysis can provide major insights into the morphologies and characteristics of cells,it may not always provide accurate quantitative data about the sizes and shapes of cells,which is critical for some applications.Although quantitative studies on the refractive indices and sizes of cells have been conducted,these investigations have typically been based on the assumption that cells are perfectly spherical.Accordingly,this assumption may not hold true for many types of cells that exhibit non-spherical shapes.Based on a texture analysis of p-Dis,this study conducted quantitative analyses on the characteristic parameters of yeast monomers and budding yeast,where the results are shown to be consistent with those obtained using traditional microscopy methods.This approach can provide insights into the quantitative analysis of non-spherical cells based on light-scattering techniques.Methods A systematic study on p-DIs with scattering angles of 60°-120°and azimuth angles of 150°-210°of 1197 yeast monomers and budding yeast was conducted using optical models established based on the discrete dipole approximation theory(DDA).Excluding the assumption that the two short aixs of yeast cells are equal,all parameters of these optical models were obtained through microscopy.The experimental p-DIs of 25000 Ale and Lager yeasts were obtained using polarization diffraction imaging flow cytometry.The Fourier spectrum and gray-level co-occurrence matrix(GLCM)parameters of all p-Dis,including those derived from a simulation and experiment,and the depolarization coefficient of p-DIs of yeast monomer were

关 键 词：生物光学 非球形生物细胞 偏振光散射 衍射成像 定量分析 

分 类 号：Q63[生物学—生物物理学]