基于激光诱导自体荧光光谱的乳腺肿瘤特异性甄别  

Specific Identification of Breast Tumors Based on Laser-Induced Autofluorescence Spectroscopy

作　　者：严剑锋 李嘉源 张幸 谭俊[1] 傅进宇 欧彩凤 张成云[1] 罗云峰[2] 陈志峰[1] 张普生[2] Yan Jianfeng;Li Jiayuan;Zhang Xing;Tan Jun;Fu Jinyu;Ou Caifeng;Zhang Chengyun;Luo Yunfeng;Chen Zhifeng;Zhang Pusheng(School of Physics and Materials Science,Guangzhou University,Guangzhou 510006,Guangdong,China;Department of Breast Surgery,Zhujiang Hospital of Southern Medical University,Guangzhou 510280,Guangdong,China;Department of Breast Care Surgery,The First Affiliated Hospital of Guangdong Pharmaceutical University,Guangzhou 510080,Guangdong,China)

机构地区：[1]广州大学物理与材料科学学院,广东广州510006 [2]南方医科大学珠江医院乳腺外科,广东广州510280 [3]广东药科大学附属第一医院乳腺科,广东广州510080

出　　处：《中国激光》2023年第21期153-158,共6页Chinese Journal of Lasers

基　　金：国家自然科学基金(11974085);广东省自然科学基金(2020A1515010411);广东省高等教育教学改革项目(2019-N466);广州市科技计划项目(2023A03J0125);北京“新锐肿瘤支持治疗课题研究”公益项目(cphcf-2022-076);广东省大学生创新训练项目(S202011078105,S202111078099)。

摘　　要：乳腺癌手术中常因缺乏快速准确的组织性质检测手段而导致组织过度切除或手术时间延长。利用组织自体光谱的检测手段因具有速度快、灵敏度高、选择性强以及无创性等优势而受到越来越多的关注。因此,针对乳腺癌寻求高效精准的光谱诊断特征并研究其内在根源具有重要意义。笔者以355 nm亚纳秒序列脉冲激光作为激发光源,采用自主搭建的激光诱导稳态荧光光谱系统对多例乳腺恶性肿瘤组织和正常乳腺组织开展自体荧光光谱实验研究。对两类组织样本的光谱特征进行比较分析,提出了基于430 nm附近光谱特征差异的特异性甄别方法,并比较了三种荧光比值法的甄别效果。进一步的光谱拟合分析揭示了实验测得的乳腺组织自体荧光主要来自4种内源性荧光物质的贡献,而癌变组织的光谱特征变化主要源于还原型辅酶Ⅰ(NADH)的增加以及NADH结合性蛋白的减少。本文提出的乳腺癌变光谱甄别方法特异性显著,生物学根源清晰,能够为临床快速检测应用提供新的技术方法参考,尤其是能为保乳手术中的切缘快速检测提供崭新的视角。Objective Breast cancer ranks highest in global incidence among all cancers,posing a significant threat to women s health.During surgical treatment for breast cancer,the absence of swift and precise tissue identification often results in either removal of excessive tissue or extended surgical durations.Recently,autologous tissue spectroscopy has garnered interest as a detection method due to its benefits,including speed,sensitivity,selectivity,and non-invasiveness.Consequently,identifying effective spectral diagnostic features for breast cancer and understanding their mechanisms are critically important.Methods When normal tissue transforms into malignant tumor tissue,there is a qualitative shift in its internal biochemical environment.Hence,when normal glandular tissue undergoes carcinogenesis,the content and distribution of various fluorescent groups within that tissue alters,causing changes in the steady-state fluorescence spectral characteristics.In this study,a custom-built laser-induced steady fluorescence spectroscopy system was employed to conduct autofluorescence spectroscopy experiments on several malignant breast tumors and normal breast tissues(Fig.1).A 355 nm sub-nanosecond sequence pulse laser served as the excitation light source.This choice was favorable for achieving a favorable signal-to-noise ratio at lower average optical power levels without inflicting irreversible damage to the samples.The constructed spectral system boasted a resolution as precise as 0.1 nm and spanned a spectral measurement range from 200 nm to 900 nm.The integration of a photomultiplier tube with a single-photon counter facilitated the measurement of faint spectral signals.Results and Discussions By comparing the spectral characteristics of the two types of tissue samples(Fig.2),the spectral curves of tumors exhibit varying degrees of concave features near 430 nm,while all spectral curves of normal breast tissue samples show no concave features around 430 nm.This stands out as the most significant spectral characteristic

关 键 词：医用光学 自体荧光 乳腺肿瘤 稳态光谱 特异性甄别 

分 类 号：TN247[电子电信—物理电子学]