飞秒激光双光子聚合制备3D水凝胶微结构及其应用  

Femtosecond Laser Two-Photon Polymerization of 3D Hydrogel Microstructures and Their Applications

作　　者：武昕宜 段琦 宾凡淳 郑美玲[1] Wu Xinyi;Duan Qi;Bin Fanchun;Zheng Meiling(Laboratory of Organic Nano-Photonics and Key Laboratory of Bionic Materials and Interface Science,Technical Institute of Physics and Chemistry,Chinese Academy of Sciences,Beijing 100190,China;University of Chinese Academy of Sciences,Beijing 101407,China)

机构地区：[1]中国科学院理化技术研究所仿生材料与界面科学重点实验室,有机纳米光子学实验室,北京100190 [2]中国科学院大学,北京101407

出　　处：《中国激光》2023年第21期182-198,共17页Chinese Journal of Lasers

基　　金：国家重点研发计划(2017YFB1104300);国家自然科学基金(61975213,61475164);中奥国际合作项目(GJHZ1720)。

摘　　要：双光子聚合加工技术是基于双光子吸收效应的一种新型的微纳制造技术,已被广泛应用于微纳光子学、微机电系统、组织工程等领域。采用双光子聚合加工技术制备的3D水凝胶微结构形貌可控,而且具有高精度、适当的刚度以及良好的生物相容性等优势,可以更好地在体外模拟体内微环境,因而在生物医学领域展现出了巨大的应用潜力。本文简要介绍了双光子聚合加工技术的原理,综述了水溶性光引发剂的研究进展,着重介绍了双光子聚合加工技术制备水凝胶的研究现状及其在仿生学、生物医学等领域的应用。Significance Extracellular matrix(ECM),which has an important impact on cell morphogenesis,adhesion,proliferation,and differentiation,is a dynamic and complex microenvironment.However,the mechanism by which cells receive and process information through ECM remains unclear.Therefore,investigating the mechanism of interaction between cells and the ECM will be of great significance in cell culture,tissue engineering,and other fields.Research has shown that the microstructure of a material surface can regulate the proliferation,differentiation,and migration of cells.Hydrogels have a certain geometric shape and moisture content similar to soft tissue,and thus can simulate the ECM in vitro as well as locate and deliver therapeutic proteins in a controlled manner as a vehicle for cell transplantation.The patterned surfaces of hydrogels,cell scaffolds,and other microstructures prepared via micro/nano fabrication technology can simulate the growth and development environment of cells in vitro,particularly in a three-dimensional(3D)environment,enabling analysis of the correlation between the shape and function of cells,as well as of the mechanism behind the interaction between cells and the matrix.Traditional methods for preparing hydrogel microstructures have shortcomings including low accuracy and difficulty in regulating the morphology,which limits their application in the future.Two-photon polymerization(TPP)technology,which uses a deep-penetrating near-infrared laser as the light source,is a new micro-nano manufacturing technology with true 3D fabrication capability and high penetration depth.It can be used to fabricate 3D micro-nano structures with arbitrary high resolution and is widely used in micro-nano photonics,micro-electromechanical systems,tissue engineering,and other fields.The 3D hydrogel microstructure prepared using TPP technology has a controllable morphology,high precision,appropriate stiffness,and good biocompatibility.This can better simulate the in vitro microenvironment required in tissue engineerin

关 键 词：材料 双光子聚合 水凝胶 生物相容性 微结构 细胞支架 组织工程 

分 类 号：V261.8[航空宇航科学与技术—航空宇航制造工程]