便携式定量相位显微成像系统设计  

Design of Portable Quantitative Phase Microscopy Imaging System

作　　者：赵英然 闫克丁 李佳一 马岩 Zhao Yingran;Yan Keding;Li Jiayi;Ma Yan(School of Intelligent Science and Information Engineering,Xi’an Peihua University,Xi’an 710125,Shaanxi,China;School of Electronic Information Engineering,Xi’an Technological University,Xi’an 710021,Shaanxi,China;School of Electrical Engineering and Information,Southwest Petroleum University,Chengdu 610500,Sichuan,China)

机构地区：[1]西安培华学院智能科学与信息工程学院,陕西西安710125 [2]西安工业大学电子信息工程学院,陕西西安710021 [3]西南石油大学电气信息学院,四川成都610500

出　　处：《激光与光电子学进展》2023年第22期185-194,共10页Laser & Optoelectronics Progress

基　　金：国家自然科学基金(11804263,11747119)。

摘　　要：为实现无标记样本的定量检测,借助NX12.0搭配相关器件自主设计了一款小型化相位显微镜。相较于市面上售价高昂的相位显微镜,所设计显微镜无需相干器件,且在满足系统分辨率的前提下,将体积缩小了约60%,大幅提高了便携性,同时成本仅需5000元左右。系统中还嵌入了自动对焦算法和基于变换域的视场校正算法,以加快检测速度和相位恢复的准确率。经测试,系统在10倍物镜下的分辨率达到了分辨率板极限2.19μm,同时随机相位板的检测表明相位恢复的准确率也达到了基本需求。除此之外,还对活细胞结构和平面玻璃的缺陷进行了测试。结果表明,所提系统不仅能对活细胞进行较好的定量测量,还可以在透明/半透明平面缺陷的检测中发挥重要作用,更是证明了这种成本低、便携性高、可实现无标记样本定量的系统设计方案的可行性。To achieve quantitative detection of unlabeled samples,a miniaturized phase microscope is independently designed using NX12.0 and related devices.Compared to the expensive phase microscopes currently on the market,the newly designed microscope has an approximately 60%smaller volume while achieving the same system resolution,significantly improving portability;it does not require coherent devices,and the cost is only approximately 5000 yuan.The system also incorporates an autofocus algorithm and a field of view correction algorithm,based on transform domain techniques,to accelerate the detection speed and accuracy of phase recovery.After testing,the resolution of the system reached the resolution board limit of 2.19μm using a 10×objective lens.Further,the detection of random phase plates indicates that the accuracy of phase recovery also meets the basic requirements.Additionally,defects in the structure of living cells and flat glass are also tested.The results indicate that the proposed system can quantitatively measure living cells and play an important role in detecting transparent/semitransparent plane defects.They also prove the feasibility of this low-cost and portable unlabeled-sample quantification system design scheme.

关 键 词：成像系统 相位显微镜 无标记检测 活细胞定量测量 平面缺陷检测 3D光固化技术 

分 类 号：O436[机械工程—光学工程]