Azotobacter inoculation can enhance the resistance of Bt cotton to cotton bollworm, Helicoverpa armigera  

作　　者：Zhuo Li Ming Zhao Lei Li Yi-Yang Yuan Fa-Jun Chen Megha N.Parajulee Feng Ge 

机构地区：[1]Institute of Plant Protection,Shandong Academy of Agricultural Sciences,Jinan,China [2]State Key Laboratory of Integrated Management of Pest Insects and Rodents,Institute of Zoology,Chinese Academy of Sciences,Beijing,China [3]Shandong Cotton Research Center,Shandong Academy of Agricultural Sciences,Jinan,China [4]Department of Entomology,Nanjing Agricultural University,Nanjing,China [5]Texas A&M University AgriLife Research and Extension Center,Lubbock,Texas,USA

出　　处：《Insect Science》2023年第6期1648-1662,共15页昆虫科学（英文版）

基　　金：supported by the Shandong Provin-cial Natural Science Foundation,China(grant no.ZR2020QC134);the National Natural Science Foun-dation of China(grant no.32202405);the State Key Laboratory of Integrated Management of Pest Insects and Rodents(grant no.IPM2001);the Agricultural Sci-entific and Technological Innovation Project of Shan-dong Academy of Agricultural Sciences(grant no.CXGC2022E04);the Shandong-Chongqing Sci-ence&Technology Cooperation Program,Shandong Technological Innovation Guidance Plan(grant no.2020LYXZ030).

摘　　要：The rising trend in the cultivation of Bacillus thuringiensis (Bt) transgenic crops may cause a destabilization of agroecosystems, thus increasing concerns about the sustainability of Bt crops as a valid pest management method. Azotobacter can be used as a biological regulator to increase environmental suitability and improve the soil nitrogen utilization efficiency of crops, especially Bt cotton. A laboratory test investigated effects on the development and food utilization of Helicoverpa armigera fed with different Cry1Ab/Cry1Ac proteins and nitrogen metabolism-related compounds from cotton (transgenic variety SCRC 37 vs non-Bt cotton cv. Yu 2067) inoculated with Azospirillum brasilense (Ab) and Azotobacter chroococcum (Ac). The findings indicate that inoculation with Azotobacter significantly decreased the partial development and food utilization indexes (pupal weight;pupation rate;adult longevity;fecundity;relative growth rate, RGR;efficiency of conversion of digested food, ECD;and efficiency of conversion of ingested food, ECI) of H. armigera fed on Bt cotton, but contrasting trends were found among these indexes in H. armigera fed on non-Bt cotton inoculated with Azotobacter, as a result of differences in Bt toxin production. Overall, the results showed that inoculation with Azotobacter had negative effects on the development and food utilization of H. armigera fed on Bt cotton, leading to enhanced target insect resistance. Presumably, Azotobacter inoculation can be used to stimulate plant soil nitrogen uptake to increase nitrogen metabolism-related compounds and promote plant growth for Bt and non-Bt cotton, simultaneously raising Bt protein expression and enhancing resistance efficacy against cotton bollworm in Bt cotton.

关 键 词：AZOTOBACTER Bt protein expression development and fecundity food utilization nitrogen metabolism-related compounds transgenic Bt cotton 

分 类 号：Q96[生物学—昆虫学]