NDUFA8 potentially rescues Wolbachia-induced cytoplasmic incompatibility in Laodelphax striatellus  

作　　者：Jie Chen Meng-Ke Wang Qi-Xian Xie Xiao-Li Bing Tong-Pu Li Xiao-Yue Hong 

机构地区：[1]Department of Entomology,Nanjing Agricultural University,Nanjing,China

出　　处：《Insect Science》2023年第6期1689-1700,共12页昆虫科学（英文版）

基　　金：supported by the National Natural Science Foundation of China(No.32020103011,32001905,and 31871976).

摘　　要：The endosymbiont Wolbachia manipulates host reproduction by several strategies, one of the most important of which is cytoplasmic incompatibility (CI). CI can be rescued when Wolbachia-infected males mate with females infected with the same Wolbachia strain. However, the potential rescue mechanism of CI in the small brown planthopper Laodelphax striatellus is unclear. In this study, comparative transcriptome analysis was applied to explore the effect of Wolbachia on L. striatellus eggs. A total of 1387 differentially expressed genes were identified. RNA interference of 7 Wolbachia-upregulated key planthopper genes reduced egg reproduction, suggesting that Wolbachia might improve fecundity in L. striatellus by affecting these 7 genes. Suppressing the expression of another upregulated gene, NDUFA8 (encoding NADH dehydrogenase [ubiquinone] 1 α subcomplex subunit 8-like) by RNA interference significantly increased the mortality of early embryos without affecting the number of deposited eggs. Wolbachia infection upregulated the mRNA level of NDUFA8, and dsNDUFA8 treatment of Wolbachia-infected females recreated CI-like symptoms, suggesting that NDUFA8 is associated with the rescue phenotype. Because all L. striatellus populations worldwide are infected with Wolbachia, NDUFA8 is a potential pest control target.

关 键 词：cytoplasmic incompatibility Laodelphax striatellus NDUFA8 reproduc-tion RESCUE WOLBACHIA 

分 类 号：Q96[生物学—昆虫学]