应用宏基因组纳米孔测序技术分析流行性腮腺炎样病例病原体  

Pathogen Analysis of Mumps-like Cases Using Metagenomic Nanopore Sequencing Technology

作　　者：韩桃利 焦洋[1] 赵剑虹[1] 张淑 顾琳[1] 赵玉倩 李臻 曹阳 张建[1] 高艳[1] 孙灵利[1] HAN Taoli;JIAO Yang;ZHAO Jianhong;ZHANG Shu;GU Lin;ZHAO Yuqian;LI Zhen;CAO Yang;ZHANG Jian;GAO Yan;SUN Lingli(Beijing Chaoyang Center for Disease Control and Prevention,Beijing 100021,China)

机构地区：[1]北京市朝阳区疾病预防控制中心,北京100021

出　　处：《病毒学报》2023年第6期1615-1622,共8页Chinese Journal of Virology

基　　金：北京市朝阳区科技计划项目(项目号:CYSF2050),题目:三代测序技术对流行性腮腺炎样病例进行宏基因组分析及腮腺炎病毒基因变异研究。

摘　　要：为了解流行性腮腺炎样病例可能的病原体,本研究采用宏基因组纳米孔技术检测22例流行性腮腺炎样病例(病例组)和17例健康人群(对照组)的腮腺管口拭子中可能的病原。针对在病例组和对照组样本测序中发现的可疑病原体,应用实时荧光PCR进行检测确认。同时应用SPSS 23.0软件对两组的检测结果进行统计分析。宏基因组测序结果发现病例组和对照组未发现有统计学差异的致病细菌。人副流感病毒3型(Human parainfluenza virus3,HPIV-3)只在病例组5例病例中检出,而人疱疹病毒7型(Human herpesvirus 7,HHV-7)分别在病例组和对照组中的4例和6例病例中检出。实时荧光PCR验证结果与宏基因组测序结果一致。进一步分析发现,病例组和对照组的HPIV-3检出率的差异具有统计学意义(χ~2=9.186,P=0.011),而HHV-7检出率无统计学意义(χ~2=0.364,P=0.393)。本研究结果提示HPIV-3可能是调查地区导致流行性腮腺炎样病例的病原体,在今后对流行性腮腺炎诊断并报告时应注意鉴别,并加强病原学监测和检测。In order to understand the possible pathogens of Mumps-like cases,this study examined the possible pathogens in parotid throat swabs from 22 Mumps-like cases(case group) and 17 healthy individuals(control group) by metagenomic nanopore sequencing technology.For the suspected pathogens found between case group and control group,real-time fluorescent quantitative polymerase chain reaction(qPCR) was used for detection and confirmation.At the same time,SPSS 23.0 software was used to statistically compare the significant difference between two groups.There was no significant difference about the detection results of pathogenic bacteria found in two groups by metagenomic sequencing.Human parainfluenza virus 3(HPIV-3) was detected only in 5 cases in case group,while human herpesvirus 7(HHV-7) was detected in 4 and 6 cases in case group and control group,respectively.Further statistical analysis revealed that the difference in the HPIV-3 detection rate between case group and control group was statistically significant (χ~2=9.186,P=0.011),while the HHV-7 detection rate was not statistically significant (χ~2=0.364,P=0.393).These findings suggested that HPIV-3 may be a potential pathogen for Mumps-like cases in the investigation area,and attention should be paid to the differential diagnosis and reporting of Mumps,and etiological surveillance and detection should be strengthened.

关 键 词：流行性腮腺炎 宏基因组 Nanopore测序 人副流感病毒3型 人疱疹病毒7型 

分 类 号：R373.16[医药卫生—病原生物学]