基于液相色谱-质谱法探究慢性光化性皮炎患者血清脂质组学特征  

Characterisation of serum lipidomic profiles in patients with chronic actinic dermatitis based on liquid chromatography-mass spectrometry

作　　者：陈荃 唐奕 李华平 吴伟鸿 邓蕙妍 陈教全 陈乐滋 李振洁 朱慧兰 Chen Quan;Tang Yi;Li Huaping;Wu Weihong;Deng Huiyan;Chen Jiaoquan;Chen Lezi;Li Zhenjie;Zhu Huilan(Department of Dermatology,Guangzhou Institute of Dermatology,Institute of Dermatology,Guangzhou Medical University,Guangzhou 510095,China;Department of Dermatology,Guangzhou First People′s Hospital,Guangzhou 510180,China;Department of Vascular Surgery and Plastic Surgery,Guangdong Provincial People′s Hospital,Guangdong Academy of Medical Sciences,Guangzhou 519041,China)

机构地区：[1]广州医科大学皮肤病研究所广州市皮肤病防治所皮肤科,广州510095 [2]广州市第一人民医院皮肤科,广州510180 [3]广东省人民医院(广东省医学科学院)血管与整形外科,广州519041

出　　处：《中华皮肤科杂志》2023年第12期1107-1114,共8页Chinese Journal of Dermatology

基　　金：广东省医学科学技术研究基金(B2023070);广州市科技计划项目(202002030474、2023A03J0943);广州市临床重大技术项目(2019ZD20)。

摘　　要：目的探讨慢性光化性皮炎(CAD)患者的血清脂质组学特征,寻找CAD的生物标志物。方法本研究为回顾性分析,于2011年4月至2021年12月在广州市皮肤病防治所收集46例CAD患者以及16例年龄、性别匹配的健康对照的血清样本,利用液相色谱-质谱法检测血清脂质组成与表达变化。采用主成分分析、偏最小二乘判别分析、正交偏最小二乘判别分析等筛选差异的生物标志物,采用受试者工作特征曲线分析筛选诊断标志物。组间年龄和性别资料的比较分别采用t检验和χ^(2)检验。结果46例CAD患者年龄30~84(60.39±10.52)岁,男41例,女5例;16例健康对照,年龄50~89(59.81±10.72)岁,男14例,女2例;两组间差异无统计学意义(年龄:t=0.19,P=0.853;性别:χ^(2)=0.03,P=0.859)。血清样本中共检测出4136种脂质分子,包含40个亚类。CAD患者和健康对照两组间存在22个差异脂质分子,包含9个亚类:甘油三酯、鞘磷脂、磷脂酰丝氨酸、磷脂酰乙醇胺、单脂肪酸甘油酯、溶血磷脂酰胆碱、己糖神经酰胺、甘油二酯、心磷脂;其中甘油三酯(37.7e)+Na、单脂肪酸甘油酯(22.3)+NH4和磷脂酰丝氨酸(18.0_18.1)+H作为诊断标志物时受试者工作特征曲线下面积均>0.8,16种脂质分子的曲线下面积>0.7。结论相较于健康对照CAD患者血清中脂质成分改变,其中甘油三酯(37.7e)+Na、单脂肪酸甘油酯(22.3)+NH4和磷脂酰丝氨酸(18.0_18.1)+H可能是较有潜力的CAD诊断价值的生物标志物。Objective To investigate serum lipidomic profiles in patients with chronic actinic dermatitis(CAD),and to search for biomarkers of CAD.Methods A retrospective analysis was conducted.Serum samples were collected from 46 patients with CAD and 16 age-and gender-matched healthy controls in the Guangzhou Institute of Dermatology from April 2011 to December 2021.Changes in serum lipid composition and expression were assessed by liquid chromatography-mass spectrometry.Principal component analysis,partial least squares discriminant analysis,and orthogonal partial least squares discriminant analysis were performed to screen differential biomarkers,and receiver operating characteristic(ROC)curve analysis was conducted to screen diagnostic markers.Comparisons of the age and gender distribution between groups were performed using t test and chi-square test,respectively.Results The 46 CAD patients were aged from 30 to 84(60.39±10.52)years,including 41 males and 5 females;the 16 healthy controls were aged from 50 to 89(59.81±10.72)years,including 14 males and 2 females;there were no significant differences in the age or gender distribution between the two groups(age:t=0.19,P=0.853;gender:χ^(2)=0.03,P=0.859).Totally,4136 lipid molecules belonging to 40 subclasses were identified in the serum samples from CAD patients as well as healthy controls.Twenty-two differential lipid molecules were identified between the CAD patients and healthy controls,belonging to 9 subclasses(triglycerides,sphingomyelin,phosphatidylserine,phosphatidylethanolamine,monofatty acid glycerides,lysophosphatidylcholine,hexose ceramide,diglycerides,and cardiolipin).When the combinations of triglycerides(37.7e)and Na,those of monoglycerides(22.3)and NH4,or those of phosphatidylserine(18.0_18.1)and H served as diagnostic markers separately,the areas under the ROC curve(AUCs)were all>0.8,and the AUCs of 16 differential lipid molecules were all>0.7.Conclusion The serum lipid composition differed between healthy controls and CAD patients,and the combinations o

关 键 词：光过敏疾患 皮炎 光变态反应 营养和代谢性疾病 代谢组学 诊断技术和方法 曲线下面积 

分 类 号：R758.1[医药卫生—皮肤病学与性病学]