西葫芦cDNA全长转录组序列分析  

Sequences Analysis of cDNA Full-length Transcriptome of Squash(Cucurbita pepo)

作　　者：张中海[1] 王美丽[1] 唐忠丽 薛诗怡 张安世[1] 雷逢进[3] 许小勇 Zhang Zhonghai;Wang Meili;Tang Zhongli;Xue Shiyi;Zhang Anshi;Lei Fengjin;Xu Xiaoyong(Jiaozuo Normal College,Jiaozuo,454002;Shanxi Facility Vegetable Collaborative Innovation Center,College of Horticulture,Shanxi Agricultural University,Taigu,030801;Institute of Cotton Research,Shanxi Agricultural University,Yuncheng,044000;Yazhou Bay Laboratory,Sanya,572025)

机构地区：[1]焦作师范高等专科学校,焦作454002 [2]山西农业大学园艺学院,山西省设施蔬菜协同创新中心,太谷030801 [3]山西农业大学棉花研究所,运城044000 [4]海南省崖州湾种子实验室,三亚572025

出　　处：《分子植物育种》2023年第24期8028-8035,共8页Molecular Plant Breeding

基　　金：国家自然科学基金项目(32260767);山西省自然科技基金项目(202103021224125)共同资助。

摘　　要：为解析西葫芦中类胡萝卜素积累的分子机制,本研究利用PacBio单分子长读长测序技术(single-molecule long-read sequencing technology,SMRT)对富含类胡萝卜素的黄皮西葫芦(21pu05)叶、雌花、雄花、未授粉果实和授粉10 d果实等组织的混合样品进行全长转录组测序分析。试验结果共获得循环一致序列(circular consensus read,CCS) 158 968条,其中全长非嵌合(full length reads non-chimeric,FLNC)序列113 284条。聚类分析获得高质量的一致序列28 383条,其中融合转录本有213个。进一步分析发现16 895个可变剪接基因位点,其中新基因位点1 734个,新转录本18 510个。序列结构预测鉴定出8 773个SSR位点、8 527个完整ORF序列,以及317个长链非编码RNA (long non-coding RNA,lncRNA)。对16 508个新转录本进行了功能注释。以上研究结果为开展西葫芦类胡萝卜素合成相关基因的克隆及分子机制的解析提供参考。In order to explore the genetic basis of the specific traits of squash(Cucurbita pepo L.),in this study,transcriptome sequencing and analysis were performed with the mixed tissues of leaves,female flowers,male flowers,unpollinated fruits and fruits at 10 d after pollination by single-molecule long-read sequencing technology(SMRT).Results shows that a total of 158 968 circular consensus reads including 113 284 full length non-chimeric(FLNC) sequences were obtained.Cluster analysis obtained 28 383 high quality consistent sequences,and 213 fusion transcripts were predicted.A total of 16 895 gene sites were detected by variable splicing analysis of these redundancy transcripts,including 1 734 new gene sites and 18 510 new transcripts.Structural analysis predicted 8 773 micrisatellites,8 527 complete ORF sequences and 317 long non-coding RNA(lncRNA),and 16 508new transcripts were functional annotated.These results laid a basis for the cloning of carotenoid synthesis related genes and the mechanism analysis of yellow pigments accumulation in zucchini.

关 键 词：黄皮西葫芦 全长转录组 可变性剪接 融合转录本 

分 类 号：S642.6[农业科学—蔬菜学]