甜瓜果肉颜色遗传分析及相关分子标记的开发  

Genetic Analysis and Development of Molecular Markers for Fruit Flesh Color of Melon (Cucumis melo L.)

作　　者：阮悦 李群[1] 周继阳 康彩彦 苏比娜·肖克来提 刘慧青 王贤磊[1] Ruan Yue;Li Qun;Zhou Jiyang;Kang Caiyan;Subina·Xiaokelaiti;Liu Huiqing;Wang Xianlei(College of Life Science and Technology,Xinjiang University,Urumqi,830046)

机构地区：[1]新疆大学生命科学与技术学院,乌鲁木齐830046

出　　处：《分子植物育种》2023年第24期8127-8133,共7页Molecular Plant Breeding

基　　金：国家自然科学基金项目(31660297)资助。

摘　　要：甜瓜果肉颜色是影响消费者选择的重要果实品质性状之一。为了研究甜瓜果肉颜色性状的遗传规律,以‘库克拜热’(绿肉)与‘西州蜜’(橙肉)构建BC1群体,‘库克拜热’与‘皇后’(橙肉)构建F2群体,利用集群分离分析(bulked segregation analysis,BSA)与简单重复序列(simple sequence repeats,SSR)分子标记相结合的方法,明确了在上述甜瓜品种中,果肉颜色受两个基因的调控,其中橙色果肉控制基因gf对白色果肉控制基因wf具有显性上位作用,绿色对白色为隐性。本研究同时开发出相应的多态性分子标记,其中分子标记SSR2021、SSR2103与gf紧密连锁,分子标记SSR2956、SSR2980与wf紧密连锁,可用于区分橙色、白色和绿色果肉甜瓜。本研究为不同果肉颜色甜瓜的选育提供一定的理论基础和技术支持。Flesh color is one of the important fruit quality characters that affect consumer choice.In order to study the inheritance of flesh color traits in melon,BC1population was developed from a cross between 'Quaker Baire'(green fleshed) and 'Xizhoumi'(orange fleshed),and F2population was developed from a cross between 'Quaker Baire' and 'Queen'(orange fleshed).Using a combination of bulked segregation analysis(BSA) and simple sequence repeats(SSR) molecular markers,we obtained that flesh color was regulated by two genes in the melon cultivars mentioned above.The gf controlling orange flesh is dominant epistatic to wf controlling white and green flesh,and green is recessive to white.The corresponding polymorphic molecular markers were developed at the same time:molecular markers SSR2021 and SSR2103 were tightly linked to gf;molecular markers SSR2956 and SSR2980 were tightly linked to wf.They could be used to distinguish orange-fleshed,white-fleshed and greenfleshed melon.This research provides theoretical basis and technical support for the breeding of melons with different flesh colors.

关 键 词：甜瓜 果肉颜色 遗传 简单重复序列(SSR) 

分 类 号：S652[农业科学—果树学]