低雌激素状态下大鼠海马及纹状体Nogo-A的表达变化  

Changes of Nogo-A expression in hippocampus and striatum of rats under low estrogen condition

作　　者：王文娟 丁雨桐 苏昊[3] 任捷 孙艳荣 王涵菲 陆嘉莉 张林倩 白钰 秦丽华[1] WANG Wen-juan;DING Yu-tong;SU Hao;REN Jie;SUN Yan-rong;WANG Han-fei;LU Jia-li;ZHANG Lin-qian;BAI Yu;QIN Li-hua(Department of Human Anatomy,Histology and Embrydog,School of Basic Medical Sciences,Peking University Health Science Center,Beijing 100083,China;Department of Obstetrics,Inner Mongolia Autonomous Region Maternal and Child Health Hospital,Hohhot 010020,China;Department of Obstetrics and Gynecology,Peking Union Medical College Hospital,Beijing 100730,China;Department of Surgery,Peking Union Medical College Hospital,Beijing 100730,China;School of Stomatology,Shanxi Medical University,Taiyuan 030001,China)

机构地区：[1]北京大学医学部基础医学院人体解剖学与组织学胚胎学系,北京100083 [2]内蒙古自治区妇幼保健院产科,呼和浩特010020 [3]中国医学科学院北京协和医学院妇产科,北京100730 [4]中国医学科学院北京协和医院外科,北京100730 [5]山西医科大学口腔医学院,太原030001

出　　处：《解剖学报》2023年第6期620-627,共8页Acta Anatomica Sinica

基　　金：国家自然科学基金(82171635);北京市自然科学基金(7232091)。

摘　　要：目的通过实验观察低雌激素状态下大鼠记忆功能的改变以及海马和纹状体Nogo-A的表达变化,阐明Nogo-A在更年期神经退行性变如记忆功能减退中可能发挥的重要作用。方法45只雌性SD大鼠分为假手术组,去卵巢组和去卵巢后雌激素治疗组,每组15只。去卵巢手术后2周开始给予药物治疗。雌激素治疗组于腹股沟皮下注射溶于无菌芝麻油的雌激素[25μg/(kg·d)]。假手术组和去卵巢组给予等量的无菌芝麻油。药物治疗6周后,通过条件恐惧训练实验观察不同组大鼠记忆功能差异,通过免疫组织化学和免疫印迹法观察Nogo-A在3组大鼠海马和纹状体的表达变化。结果与假手术组和雌激素治疗组相比,去卵巢组大鼠出现记忆功能明显减退,其海马和纹状体Nogo-A阳性神经元数量显著增加(P<0.05)。雌激素治疗组大鼠记忆功能明显改善,海马和纹状体Nogo-A阳性神经元数量显著减少(P<0.05),与假手术组差异不明显(P>0.05)。免疫印迹实验结果与上述免疫组织化学结果变化趋势一致。结论低雌激素状态下海马及纹状体Nogo-A表达上调,这可能是更年期妇女记忆功能减退的重要原因之一。Objective To elucidate the important role of Nogo-A in climacteric neurodegeneration such as memory impairment by observing memory function and the expression of Nogo-A in hippocampus and striatum of rats under low estrogen condition. Methods Fouthy-five female SD rats were divided into sham operation group, ovariectomized group and ovariectomized estrogen treatment group with 15 rats in each group. Medication was given 2 weeks after ovariectomized. Estrogen treatment group was subcutaneously injected in groin with estrogen [25 μg/(kg·d)] dissolved in sterile sesame oil. The sham operation group and the ovariectomized group were given the same amount of aseptic sesame oil. Samples were collected after 6 weeks of drug treatment. The difference of memory function of rats in three groups was observed by conditioned fear training experiment, and the expression of Nogo-A in hippocampus and striatum was observed by immunohistochemistry and Western blotting. Results Compared with the sham and estrogen treatment group, memory function in ovariectomized group decreased significantly and the number of Nogo-A positive neurons in hippocampus and striatum of ovariectomized rats was significantly higher than that of sham operation group(P< 0.05). There was no difference between the estrogen treatment group and the sham operation group with the memory function and the Nogo-A expression(P > 0.05). The result of immunoblotting was consistent with the above-mentioned immunohistochemical result. Conclusion The increased expression of Nogo-A in hippocampus and striatum under low estrogen condition may be one of the key reasons for memory impairment in climacteric women.

关 键 词：低雌激素 海马 纹状体 NOGO-A 记忆功能 免疫印迹法 大鼠 

分 类 号：R338.2[医药卫生—人体生理学]