白细胞介素6对脂多糖诱导所致类子痫前期大鼠有核红细胞的影响  

Effect of interleukin-6 on nucleated erythrocytes in lipopolysaccharide- induced preeclampsia rats

作　　者：刘丽平 朱梦妮 徐慧[2] LIU Li-ping;ZHU Meng-ni;XU Hui(Department of Obstetrics of the Sixth Hospital of Wuhan,Affiliated Hospital of Jianghan University,Wuhan430015,China;Department of Obstetrics of Wuhan Children’s Hospital(Wuhan Maternal and Child Healthcare Hospital),Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430015,China)

机构地区：[1]江汉大学附属医院武汉市第六医院产科,武汉430015 [2]华中科技大学同济医学院附属武汉儿童医院(武汉市妇幼保健院)产科,武汉430015

出　　处：《解剖学报》2023年第6期722-729,共8页Acta Anatomica Sinica

基　　金：武汉儿童医院科研计划项目(2021FE010)。

摘　　要：目的探讨白细胞介素(IL)-6对脂多糖(LPS)诱导类子痫前期大鼠有核红细胞的影响。方法采用ELISA和免疫组织化学法检测正常妊娠、轻和重度子痫前期患者外周血与胎盘中IL-6的表达;采用流式细胞术和免疫荧光法检测母体有核红细胞比例。SD妊娠大鼠随机分为3组:对照组、LPS组和LPS+anti-IL-6组,检测外周血和胎盘中IL-6、有核红细胞比例、JAK2/MEK和PI3K/Akt信号基因表达。结果子痫前期患者的IL-6明显高于正常妊娠者。相比于对照组,LPS组外周血和胎盘中IL-6、有核红细胞比例和JAK2、P85、Akt、P65、IL-1B mRNA表达明显增加,胎鼠体重明显降低;相比于LPS组,LPS+anti-IL-6组外周血和胎盘中IL-6表达、有核红细胞比例、JAK2、P85、Akt、P65和IL-1B mRNA表达明显减少。结论子痫前期患者外周血和胎盘IL-6上调伴随外周血有核红细胞增多,在体中和IL-6可能通过下调有核红细胞JAK2/PI3K/Akt/NF-κB信号介导的IL-1B表达从而保护类子痫前期大鼠。Objective To explore the effect of interleukin(IL)-6 on nucleated erythrocytes in lipopolysaccharide(LPS)-induced preeclampsia rats. Methods ELISA and immunohistochemistry were used to detect the IL-6 in peripheral blood and placenta of preeclampsia and normal pregnancy;Flow cytometry and immunofluorescence were used to detect the maternal nucleated erythrocytes. Pregnant SD rats were randomly divided into 3 groups: the control, LPS and LPS +anti-IL-6 group;IL-6, the proportion of nucleated erythrocytes, JAK2/MEK and PI3K/Akt signal-related genes were detected. Results The IL-6 of preeclampsia was higher than that of normal patients. Compared with the Control group, IL-6, the proportion of nucleated erythrocytes and JAK2, P85, Akt, P65, IL-1B mRNA of LPS group increased, the fetal weight decreased;Compared with the LPS group, IL-6, the proportion of nucleated erythrocytes and JAK2, P85, Akt, P65 and IL-1B mRNA of the LPS+anti-IL-6 group decreased. Conclusion The up-regulation of IL-6 of preeclampsia patients is accompanied by increased nucleated erythrocytes in peripheral blood. Neutralizing IL-6 in vivo may down-regulate JAK2/PI3K/Akt/NF-κB-signal-mediated IL-1B to protect preeclampsia rats.

关 键 词：子痫前期 白细胞介素6 有核红细胞 白细胞介素1B 免疫组织化学 免疫荧光 人 大鼠 

分 类 号：R285.5[医药卫生—中药学]