禽白血病p27蛋白冻干粉标准物质研制  

Development of avian leukosis virus p27 protein lyopgilized powder reference material

作　　者：赵旭 石莲花 武利庆 金有训 ZHAO Xu;SHI Lianhua;WU Liqing;JIN Youxun(National Institute of Metrology,China,Beijing 100029,China)

机构地区：[1]中国计量科学研究院,北京100029

出　　处：《中国测试》2023年第11期166-175,共10页China Measurement & Test

基　　金：国家重点研发计划项目(2022YFC2009600,2022YFC2009602)。

摘　　要：为了临床检验中p27蛋白含量测定结果的量值溯源,制备禽白血病p27蛋白冻干粉标准物质。建立同位素稀释质谱法对纯品禽白血病p27蛋白含量进行测定,结果表明,该方法准确、可靠,可以作为p27蛋白标准物质的定值方法。同时结合多家实验室定值,最终定值结果为(1.46±0.13)mg/mL。该标准物质均匀性良好,在–20℃、4℃和室温环境下可以稳定7天,在–70℃环境中可以保存6个月。禽白血病p27蛋白冻干粉标准物质的研制对禽白血病检测的质量控制、不同检测方法间的评价具有很好的实用价值。In order to meet the requirements of traceability of clinical p27 protein measurement results,reference materials of avian leukosis virus p27 protein lyophilized powder were developed.The value of the reference materials of avian leukosis virus p27 protein lyophilized powder were evaluated by HPLC-IDMS/MS in several laboratories,and the certified values were(1.46±0.13)mg/mL.The developed method is accurate and reliable,it is able to be applied for the development of p27 protein reference material.The result indicated that the reference materials were sufficient homogeneity and had good stability,could be stored for up to 7 days at–20℃,4℃and room temperature.It could be stored for 6 months at–70℃.The development of p27 protein reference material is important for quality control of avian leukemia assays and for the evaluation of different avian leukemia assays.

关 键 词：禽白血病 P27蛋白 同位素稀释质谱法 标准物质 

分 类 号：S855.3[农业科学—临床兽医学] TB9[农业科学—兽医学]