嵌套EP管法更快速获取更多小鼠骨髓细胞  

Tube-nesting EP:A new faster method to get more mouse bone marrow cells

作　　者：徐启英 王卓亚[1,3] 冶怡 乌仁塔娜[1] XU Qiying;WANG Zhuoya;YE Yi;WUREN Tana(Plateau Medicine Research Center,Qinghai University,Key Laboratory of Plateau Medicine of the Ministry of Education,Qinghai Provincial Key Laboratory of Plateau Medicine Application Foundation,Qinghai-Utah Joint Key Laboratory of Plateau Medicine,Xining 810001,China)

机构地区：[1]青海大学高原医学研究中心,高原医学教育部重点实验室,青海省高原医学应用基础重点实验室,青海-犹他高原医学联合重点实验室,西宁810001 [2]青海大学附属医院妇科,西宁810001 [3]青海大学附属医院老年二科,西宁810001

出　　处：《中国免疫学杂志》2023年第12期2624-2626,2630,共4页Chinese Journal of Immunology

基　　金：青海省科协中青年科技人才托举工程项目(2022QHS⁃KXRCTJ44);青海省高等学校省级骨干教师培养对象项目。

摘　　要：目的:探讨更快速获取更多小鼠骨髓细胞的方法。方法:取同一只小鼠双下肢,两侧分别随机使用首创的嵌套EP管法或传统常用的注射器冲洗法获得骨髓细胞;记录获取骨髓细胞的时间、数量;采用流式细胞技术分析两种方法所得的活细胞比例;采用CCK-8方法检测两种方法获得的小鼠骨髓细胞的增殖能力。结果:与传统常用的注射器冲洗法相比,嵌套EP管法获取小鼠骨髓细胞的时间更短(P<0.0001)。与传统常用的注射器冲洗法相比,嵌套EP管法获得的小鼠骨髓细胞数量更多(P<0.05)。两种方法获得的骨髓活细胞比例一致,增殖能力一致。结论:嵌套EP管法能更快速获得更多的骨髓细胞。Objective:To explore a method to obtain more mouse bone marrow cells more quickly.Methods:Take both lower limbs of the same mouse,the bone marrow cells were obtained by either the tube-nesting EP method or the traditional syringe irrigation method,recorded the time and quantity of bone marrow cells,to analyze the proportion of viable cells by flow cytometry,and to detect the proliferation of these bone marrow cells used CCK-8 method.Results:Compared with the traditional syringe irrigation method,the time of obtaining mouse bone marrow cells by tube-nesting EP method was shorter(P<0.0001).Compared with the traditional syringe irrigation method,the number of bone marrow cells obtained by tube-nesting EP method was more(P<0.05).The proportion of live cells and proliferation ability of bone marrow cells obtained by the two methods were identical.Conclusion:Tube-nesting EP method is a new faster method to get more mouse bone marrow cells.

关 键 词：嵌套EP管法 骨髓细胞 小鼠 

分 类 号：Q34[生物学—遗传学]