单碱基编辑技术在动物中的应用  

Application of single base gene editing technology in animals

作　　者：陈力 王静 吴平先 张廷焕 郭宗义[1,2] 王金勇 CHEN Li;WANG Jing;WU Pingxian;ZHANG Tinghuan;GUO Zongyi;WANG Jinyong(Chongqing Academy of Animal Science,Chongqing 402460,China;National Center of Technology Innovation for Pigs,Chongqing 402460,China)

机构地区：[1]重庆市畜牧科学院,重庆402460 [2]国家生猪技术创新中心,重庆402460

出　　处：《黑龙江畜牧兽医》2023年第23期21-25,31,130-132,共9页Heilongjiang Animal Science And veterinary Medicine

基　　金：重庆市人民政府与中国农业科学院战略合作项目(23310);国家现代农业产业技术体系项目(CARS-35);重庆市自然科学基金面上项目(cstc2021jcyj-msxmX0784)。

摘　　要：近年发展起来的人工核酸酶技术可引起特定位点的DNA双链断裂,断裂后通过同源重组修复机制使精准单碱基编辑成为可能。基于CRISPR/Cas9将Cas9n与胞嘧啶脱氨酶或腺苷脱氨酶融合而开发出的单碱基编辑系统可进一步提高单碱基编辑的效率和精确度,在不造成DNA双链断裂的情况下实现精准单碱基编辑。对动物进行单碱基编辑不仅有望提高其生产性能,而且能为人类疾病研究作出重要贡献。文章回顾了单碱基编辑系统的发展历程,重点介绍了单碱基编辑系统在动物中的应用,并对单碱基编辑系统在动物中的应用前景进行了展望,以期为动物基因编辑技术的研发和应用提供理论依据和应用案例。Recently developed artificial nuclease technology can cause DNA double strand breaks at specific sites,and after the break,precise single base editing is possible through homologous recombination repair mechanism.The single base gene editing system developed by fusing Cas9n with cytosine or adenosine deaminase based on CRISPR/Cas9 can further improve the efficiency and accuracy of single base gene editing,and achieve precise single base editing without causing double strand breaks in DNA.Single-base gene editing in animals is not only expected to improve their production performance,but also to make important contributions to human disease research.This paper reviewed the development history of single-base gene editing system,focused on the application of single-base editing system in animals,and prospected the application prospect of single-base editing system in animals,in order to provide theoretical basis and application cases for the development and application of animal gene editing technology.

关 键 词：同源重组 单碱基编辑系统 胞嘧啶碱基编辑器 腺嘌呤碱基编辑器 糖基化酶碱基编辑器 引导编辑器 

分 类 号：S811[农业科学—畜牧学] Q344.13[农业科学—畜牧兽医]