高效液相色谱-电感耦合等离子体质谱法测定PM_(2.5)中硒形态  

Determination of Selenium Species in PM_(2.5) by High Performance LiquidChromatography-Inductively Coupled Plasma Mass Spectrometry

作　　者：周闰[1] 孟元华[1] 曹修玉 徐志飞[1] 龚燕[1] ZHOU Run;MENG Yuan-hua;CAO Xiu-yu;XU Zhi-fei;GONG Yan(Wuxi Center for Disease Control and Prevention,Wuxi,Jiangsu 214023,China)

机构地区：[1]无锡市疾病预防控制中心,江苏无锡214023

出　　处：《环境监测管理与技术》2023年第6期57-60,共4页The Administration and Technique of Environmental Monitoring

基　　金：无锡市科协软课题基金资助项目(KX-23-C134);无锡市公共卫生中心课题基金资助项目(LCZX2021006)。

摘　　要：石英空气滤膜样品以5 mmol/L柠檬酸为提取剂,75℃水浴超声浸提3 h,浸提液用高效液相色谱-电感耦合等离子体质谱法(HPLC-ICP/MS)测定硒酸根[Se(Ⅵ)]、亚硒酸根[Se(Ⅳ)]、硒脲(SeUr)、硒代蛋氨酸(SeMet)等4种硒形态。结果表明,方法在0μg/L~50.0μg/L范围内线性良好,上述4种硒形态方法检出限分别为0.25μg/L、0.58μg/L、1.17μg/L、0.25μg/L。空白滤膜加标样11次测定结果的RSD为1.1%~4.9%,平均加标回收率为70.0%~103%。将该方法用于分析无锡地区PM_(2.5)中的硒,发现主要是无机硒形态,未检出有机硒形态。The samples collected by quartz filter membrane were extracted by ultrasound in 75℃water bath for 3 h,using 5 mmol/L citric acid as the extraction agent.Selenate[Ⅵ],selenite[Ⅳ],selenourea(SeUr)and selenomethionine(SeMet)in the extraction solution were determined by high performance liquid chromatography-inductively coupled plasma mass spectrometry(HPLC-ICP/MS).The results showed that the method had good linearity in the range of 0μg/L to 50.0μg/L,and the method detection limits for the above four selenium species were 0.25μg/L,0.58μg/L,1.17μg/L and 0.25μg/L,respectively.The RSD of 11 measurements of spiked blank filter membrane samples ranged from 1.1%to 4.9%,and the average recoveries ranged from 70.0%to 103%.The method was applied in analyzing selenium in PM_(2.5) in Wuxi area,inorganic selenium was found to be dominant,but organic selenium was not detected.

关 键 词：硒 形态分析 高效液相色谱-电感耦合等离子体质谱法 PM_(2.5) 

分 类 号：O657.63[理学—分析化学] O657.72[理学—化学]