聚丙烯腈/马齿苋提取物伤口敷料的制备及抗菌性能  

Preparation and Antibacterial Properties of Polyacrylonitrile/Portulaca oleracea L.Extract Wound Dressing

作　　者：王译泽 陈鹤鸣 晁小珊 胡娇 康花 王颖 WANG Yize;CHEN Heming;CHAO Xiaoshan;HU Jiao;KANG Hua;WANG Ying(College of Textile and Fashion Design,Xinjiang University,Urumqi 830046,China;College of Life Science and Technology,Xinjiang University,Urumqi 830046,China)

机构地区：[1]新疆大学纺织与服装学院,乌鲁木齐830046 [2]新疆大学生命科学与技术学院,乌鲁木齐830046

出　　处：《功能高分子学报》2023年第6期578-586,共9页Journal of Functional Polymers

基　　金：自治区自然科学基金面上项目(2021D01C059);新疆大学博士科研启动基金项目(2019720001)。

摘　　要：以聚丙烯腈(PAN)为原料、N,N-二甲基甲酰胺(DMF)为溶剂,通过溶液静电纺丝法制备了一种负载马齿苋提取物(POE)的PAN/POE纳米纤维膜。利用扫描电子显微镜(SEM)、红外光谱、接触角等实验对纳米纤维膜的形貌、化学结构、力学性能、润湿性能、体外释放性能、抗氧化性和抗菌性能进行了表征,并对PAN/POE纳米纤维膜的抑菌机制进行了初步探究。结果表明:随着静电纺丝液中POE质量分数(w(POE))的增加,PAN/POE纳米纤维膜的纤维直径逐渐增加,当w(POE)=12%时,纤维直径高达(397.40±71.36)nm;纳米纤维膜的力学性能随着w(POE)的增加而增大,PAN/POE的断裂强度高达(11.76±0.46)MPa。此外,纳米纤维膜中POE的累计释放量随着w(POE)的增大而增大,最高可达(93.59±2.22)%,且对2,2-二苯基-1-苦肼基(DPPH)自由基清除率最高可达(87.08±4.43)%。同时,PAN/POE对金黄色葡萄球菌和大肠杆菌均有较强的抑制作用,抑菌圈直径最高可达(12.42±1.70)mm和(16.76±0.49)mm。荧光染色实验和SEM结果表明,POE能够破坏细菌细胞膜,从而达到杀菌效果。Portulaca oleracea L.is a common plant which has received a lot of attention due to its superior antimicrobial properties.A nanofiber membrane loaded with Portulaca oleracea L.extract(POE)was prepared by electrospinning method using polyacrylonitrile(PAN)as raw material and N,N-dimethylformamide(DMF)as solvent.The nanofiber membrane morphology,chemical structure,mechanical properties,wettability,in vitro release properties,antioxidant and antimicrobial properties were characterized,and the antibacterial mechanism of PAN/POE nanofiber membranes was initially investigated.Results showed that the fiber diameter of the PAN/POE nanofiber membrane increased gradually with the increase of extract concentration,and the diameter was as high as(397.40±71.36)nm at POE mass fraction(w(POE))of 12%.The mechanical properties of the nanofiber membrane increased with the increase of w(POE),and the fracture strength was up to(11.76±0.46)MPa.In addition,the cumulative release of POE in the nanofiber membrane increased with the increase of w(POE),up to(93.59±2.22)%.The scavenging rate of 2,2-diphenyl-1-picrylhydrazyl(DPPH)radicals was up to(87.08±4.43)%.The inhibition circle diameters of Staphylococcus aureus and Escherichia coli were up to(12.42±1.70)mm and(16.76±0.49)mm,respectively.POE is able to destroy the bacterial cell membranes and thus achieve the bactericidal effects through fluorescence staining experiments and SEM observations.The PAN/POE nanofiber membrane has great potential in the field of wound dressing.

关 键 词：静电纺丝 马齿苋提取物 聚丙烯腈 伤口敷料 抗菌性能 

分 类 号：R318.08[医药卫生—生物医学工程] TB34[医药卫生—基础医学]