中国南方稻田及其周边环境根结线虫种类鉴定  

Identification of root-knot nematode species from paddy fields and surrounding environment in southern China

作　　者：徐幸 卓富彦[2] 杨芳[1] 袁斌 彭云良[1] 郭荣[2] 姬红丽[1] XU Xing;ZHUO Fuyan;YANG Fang;YUAN Bin;PENG Yunliang;GUO Rong;JI Hongli(Key Laboratory of Integrated Management of Pests on Crops in Southwest China,Ministry of Agriculture and Rural Affairs,Institute of Plant Protection,Sichuan Academy of Agricultural Sciences,Chengdu 610066,China;National Agro-Tech Extension and Service Center,Beijing 100125,China;Key Laboratory of Integrated Pest Management on Crops in Central China,Ministry of Agriculture and Rural Affairs,Wuhan 430064,China)

机构地区：[1]农业农村部西南有害生物综合治理重点实验室,四川省农业科学院植物保护研究所,成都610066 [2]全国农业技术推广服务中心,北京100125 [3]农业农村部华中作物有害生物综合治理重点实验室,武汉430064

出　　处：《植物保护》2023年第6期95-103,共9页Plant Protection

基　　金：四川省农业科学院“1+9”揭榜挂帅科技攻关项目(1+9KJGG006);国家水稻产业技术体系(CARS-01-45);四川省农业科学院科技成果中试熟化示范工程项目(CGZH2019GH03);农业部华中作物有害生物综合治理重点实验室/农作物重大病虫草防控湖北省重点实验室开放基金课题(2020ZTSJJ4);湖北省农业科技创新中心项目(2020-620-003-001)。

摘　　要：为了解根结线虫在中国南方稻区的分布和种类,从15个省(直辖市/自治区)采集1181份土样,经生物测定发现312份土样中种植的水稻或番茄幼苗根系会产生根结,阳性总检出率为26.42%。利用线虫特异性引物从171份阳性样品中鉴定出6种根结线虫:拟禾本科根结线虫Meloidogyne graminicola、北方根结线虫M.hapla、花生根结线虫M.arenaria、爪哇根结线虫M.javanica、象耳豆根结线虫M.enterolobii和南方根结线虫M.incognita,分别占鉴定总样品数的88.89%、41.52%、29.82%、10.53%、4.09%和3.51%。被1、2、3、4、5、6种根结线虫混合侵染的土样比例分别为42.11%、41.52%、12.87%、2.92%、0.58%和0,其中最常见的为拟禾本科根结线虫单独侵染(34.50%)、拟禾本科根结线虫与北方根结线虫(22.81%)或花生根结线虫(13.45%)混合侵染。本研究结果将为制定相应的传播阻断策略和防控措施提供参考。To investigate the root-knot nematode(RKN)species in rice production areas in southern China,1181 soil samples were taken from 15 provinces(municipality/autonomous region).Totally 312 samples were confirmed to form root galls on the roots of rice or tomato.The positive ratio was 26.42%.Six species of RKNs were identified from 171 soil samples by PCR amplification using specific primers.In the identified samples,the detection ratio of Meloidogyne graminicola,M.hapla,M.arenaria,M.javanica,M.enterolobii,and M.incognita was 88.89%,41.52%,29.82%,10.53%,4.09%and 3.51%,respectively.The ratio of soil samples infested by one,two,three,four,five and six species of RKNs was 42.11%,41.52%,12.87%,2.92%,0.58%and 0,respectively.The most common infestation in all soil samples was by M.graminicola alone(34.50%),followed by mixed infestation with M.hapla(22.81%)and M.arenaria(13.45%).Our study provides valuable references for the prevention and control of rice root-knot nematodes.

关 键 词：稻田 拟禾本科根结线虫 混合侵染 生物测定 特异性引物 

分 类 号：S432.45[农业科学—植物病理学]