一种用玉米拟轮枝镰孢穗腐病病粒制备接种体的简易方法  

A simple method for preparing inoculum from diseased grains of Fusarium ear rot in maize

作　　者：孙静[1] 谢淑娜[1] 李保叶 钱蕾[2] 茹艳艳[1] 刘桂珍 郝俊杰[1] SUN Jing;XIE Shuna;LI Baoye;QIAN Lei;RU Yanyan;LIU Guizhen;HAO Junjie(Institute of Plant Protection,Henan Academy of Agricultural Sciences,Key Laboratory of Integrated Pest Management on Crops in Southern Region of North China,Ministry of Agriculture and Rural Affairs,Henan Key Laboratory for Control of Crop Diseases and Insect Pests,Zhengzhou 450002,China;Zhengzhou Agricultural Technology Extension Center,Henan Province,Zhengzhou 450000,China;Henan Seed Industry Development Center,Zhengzhou 450046,China)

机构地区：[1]河南省农业科学院植物保护研究所,农业农村部华北南部作物有害生物综合治理重点实验室,河南省农作物病虫害防治重点实验室,郑州450002 [2]河南省郑州市农业技术推广中心,郑州450000 [3]河南省种业发展中心,郑州450046

出　　处：《植物保护》2023年第6期223-230,共8页Plant Protection

基　　金：河南省农业科学院自主创新项目(2023ZC050);2022年度河南省科技研发计划联合基金(222301420108);河南省农业科学院科技创新团队专项基金(2023TD30)。

摘　　要：制备接种体是玉米穗腐病抗性鉴定中的一个重要环节,本研究提出了一种用玉米拟轮枝镰孢穗腐病病粒制备接种体的简易方法,即将上年的拟轮枝镰孢穗腐病带菌病粒按一定重量加入无菌水洗脱制成接种体。田间接种试验结果表明,采用本方法简易制备的接种体接种后可获得与常规制备接种体相当的接种效果,且在-18℃冻存15 d仍可达到新鲜的常规制备接种体接种后的水平。使用简易制备接种体对33个玉米品种进行了田间穗腐病抗性鉴定,21个品种表现为感病或高感。与实验室常规培养制备接种体的方法相比,本制备方法简便易操作,用时短,成本低,无需专业仪器设备,能够一次制备大量接种体,可广泛用于玉米种质材料和新品种的拟轮枝镰孢穗腐病田间抗性鉴定及抗性材料筛选等。Preparation of inoculum is an important part of the disease resistance identification to maize ear rot.In this study,we proposed a simple method for the preparation of inoculum for maize ear rot caused by Fusarium verticillioides,in which the maize grains of last year infected by F.verticillioides were eluted with sterile water according to a certain weight to prepare the inoculum.Field inoculation experiments showed that the inoculation effect of the simply prepared inoculum was equivalent to that of conventionally prepared inoculum,even when the former was frozen at-18℃for 15 days.Using simply prepared inoculum,33 maize cultivars were identified for resistance to Fusarium ear rot,and 21 cultivars were susceptible or highly susceptible.Compared with the conventional laboratory method for preparation of Fusarium ear rot inoculum,this method is simple,easy to operate,short in time,low in cost,no need for professional equipment and the prepared inoculation volume is large.The simple preparation method can be widely used for field resistance identification and screening of resistance materials of germplasm materials and new varieties of maize.

关 键 词：玉米穗腐病 拟轮枝镰孢 接种体 

分 类 号：S435.13[农业科学—农业昆虫与害虫防治]