樟子松固沙林地可培养溶磷细菌的筛选及鉴定  

Screening and Identification of Phosphate-Solubilizing Bacteria from Pinussylvestris var. mongolica Plantation

作　　者：李建男 郝若尧 樊丽婷 李玉萌 张胜男 孟建宇[1] LI Jiannan;HAO Ruoyao;FAN Liting;LI Yumeng;ZHANG Shengnan;MENG Jianyu(Laboratory for Environmental Microbiology and Biotechnology in Arid and Cold Regions,College of Life Sciences,Inner Mongolia Agricultural University,Hohhot,Inner Mongolia 010010,China;Inner Mongolia Academy of Forestry,Hohhot,Inner Mongolia 010010,China)

机构地区：[1]内蒙古农业大学生命科学学院旱寒区环境微生物学与技术实验室,内蒙古呼和浩特010011 [2]内蒙古自治区林业科学研究院,内蒙古呼和浩特010010

出　　处：《天津农业科学》2023年第11期6-10,共5页Tianjin Agricultural Sciences

基　　金：内蒙古自治区自然科学基金(2018MS03042、2022MS03025);内蒙古自治区应用技术研究与开发资金(2021GG0360);内蒙古农业大学生命科学学院科研创新团队项目(202104)。

摘　　要：磷是植物生长发育不可缺少的营养元素,但在土壤中很难被植物直接利用,从而限制了植物的生长,而土壤中的溶磷菌可以促进植物对磷的吸收与利用。本研究旨在通过研究土壤中溶磷菌的种类,从而为微生物肥料菌种的资源库以及微生物解磷菌剂的开发和生产提供菌种资源。本研究通过纯培养方法以及选择性培养基对从樟子松固沙林土壤分离出的解磷细菌的溶磷能力进行定性分析,钼锑抗比色法对其进行定量分析,并通过细菌基因组DNA的提取以及16S rDNA扩增、测序、比对进行鉴定。结果表明:从樟子松固沙林土壤中筛选出3株溶磷能力较强的菌株,编号分别为30-3、30-4和36-5;其近缘物种分别为Bacillus aryabhattai、Paenibacillus tyrfis和Rhizobium tropici,溶磷量分别为37.63、37.63、32.24 mg·L^(-1)。由此可知,樟子松固沙林土壤中具有较多种类的溶磷菌,并且溶磷能力较强,可为后续微生物肥料的开发以及研制提供种质资源参考。Phosphorus is an indispensable nutrient element for plant growth and development,but phosphorus is difficult to be directly utilized by plants in the soil,thus limiting plant growth,and the presence of phosphorus-solubilizing bacteria in the soil can promote the uptake and utilization of phosphorus by plants.The aim of this study was to study the species of phosphorus solubilizing bacteria in soil,so as to provided strain resources for the resource bank of microbial fertilizer strains as well as the development and production of microbial phosphorus solubilizing bacterial agents.In this study,the phosphorus solubilizing ability of the phosphate solubilizing bacteria isolated from the soil of Zhangzi pine sand fixation forest was qualitatively analyzed by pure culture method and selective medium,quantitatively analyzed by molybdenum antimony antimony colorimetric method,and identified by the extraction of bacterial genomic DNA,and by the amplification,sequencing,and comparison of 16S rDNA.The results showed that three strains with strong phosphorus solubilizing capacity were screened out from the soil of camphor pine sand fixation forest with the numbers of 30-3,30-4 and 36-5,and their close relatives were Bacillus aryabhattai,Paenibacillus tyrfis and Rhizobium tropici,and the phosphorus solubilizing amount was 37.63 mg·L^(-1),37.63 mg·L^(-1) and 32.24 mg·L^(-1),respectively.It could be seen that there are more species of phosphorus solubilizing bacteria in the soil of Zhangzi pine sand fixation forest and their phosphorus solubilizing capacity is strong,which could provide germplasm resources for the subsequent development of microbial fertilizers.

关 键 词：溶磷细菌 固沙林 樟子松 16S rDNA 

分 类 号：S718.83[农业科学—林学]