泡核桃青果贮藏期间生理指标响应  

Response of Physiological Index during the Storage of Juglans sigillata Dode.Walnut with Husk

作　　者：梁美 王纪辉 胡伯凯 刘亚娜 耿阳阳 张时馨 曾亚军 何佳丽 杨光 Liang Mei;Wang Jihui;Hu Bokai;Liu Yana;Geng Yangyang;Zhang Shixin;Zeng Yajun;He Jiali;Yang Guang(Guizhou Institute of Walnut,Guiyang 550005,China;Guizhou Academy of Forestry,Guiyang 550005,China;Guizhou Sunshine Food Company Limited,Bijie 551600,China)

机构地区：[1]贵州省核桃研究所,贵州贵阳550005 [2]贵州省林业科学研究院,贵州贵阳550005 [3]贵州阳光食品有限公司,贵州毕节551600

出　　处：《南京师大学报（自然科学版）》2023年第4期21-28,共8页Journal of Nanjing Normal University(Natural Science Edition)

基　　金：贵州省科技计划重点项目(黔科合支撑[2022]重点014号);贵州省木本粮油加工科技创新人才团队平台项目(黔科合平台人才[2020]5003);贵州省林业厅优秀青年人才培养专项资金项目(黔林科合J[2018]16号).

摘　　要：解析核桃青果低温贮藏期间氧化状态.以“泡核桃”为试材,研究在冷藏过程中核桃青果的超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)、脂氧合酶(LOX)、多酚氧化酶(PPO)活性及丙二醛(MDA)、可溶性蛋白质(SP)、还原糖(RS)、维生素E(VE)、类黄酮、总酚(TP)含量变化.结果表明,SOD、POD活性呈先升高后降低的趋势走向,贮藏10 d时酶活性较高为[(858.38±27.41)U/g鲜重]、[(142.70±4.29)U/g鲜重],较70 d时降低44.40%、54.02%.CAT、LOX、PPO活性呈现波浪形变化,在贮藏40 d时CAT活性较高为[(809.12±43.20)nmol·(min·g)^(-1)鲜重],在贮藏20 d时LOX活性较高为[(161.74±4.05)U/g鲜重],在贮藏10 d时PPO活性较高为[(66.97±4.81)U/g鲜重];MDA含量缓慢增加,RS含量呈升高-下降的趋势变化,在贮藏10 d时RS含量较高为[(69.39±2.34)mg/g鲜重],在贮藏10 d时VE含量较高为[(619.47±38.46)μg/g].SP含量呈先下降再升高的趋势变化,类黄酮、总酚(TP)含量变化较为一致均呈“N”字型走向;相关性分析表明,贮藏时间与SOD、RS呈现出极显著性负相关关系(r=-0.91、-0.90,P<0.01),贮藏时间与MDA、SP则呈极显著性正相关关系(r=0.94、0.89,P<0.01),部分指标呈显著/极显著性正负相关;主成分分析结果表明,SOD、POD、CAT、LOX等众多生理指标与果实品质密切相关,可作为评价其氧化程度的重要指标.以上结果可为民间冷库贮藏青核桃提供基础数据.The oxidation state of walnut with husk was analyzed during the low-temperature storage.Juglans sigillata Dode was used as the test material,the superoxide dismutase(SOD),peroxidase(POD),catalase(CAT),lipoxygenase(LOX),polyphenol oxidase(PPO)activity and malondialdehyde(MDA),soluble protein(SP),reducing sugar(RS),VE,flavonoids,total phenol(TP)content changes were studied.The results showed that SOD and POD activity increased firstly and then decreased,and the enzyme activity was higher at 10 d of storage that was[(858.38±27.41)U/g fresh weight],and[(142.70±4.29)U/g fresh weight],which was reduced by 44.40%and 54.02%compared with 70 d.CAT,LOX,and PPO activity showed wavy changes,the CAT activity was higher at 40 d of storage that was[(809.12±43.20)nmol·(min·g)^(-1)fresh weight],the LOX activity was higher at 20 d of storage that was[(161.74±4.05)U/g fresh weight],the PPO activity was higher at 10 d of storage that was[(66.97±4.81 U/g fresh weight];The MDA content was slowly increased,the RS content was higher at 10 d of storage that was[(69.39±2.34)mg/g fresh weight],the VE content was higher at 10 d of storage that was[(619.47±38.46)μg/g].The SP content showed a trend of decreasing firstly,and then increasing,and the content of flavonoids and total phenol(TP)showed a trend change of"N"type;Correlation analysis showed that storage time showed a very significant negative relationship with SOD and RS(r=-0.91,-0.90,P<0.01),and storage time showed a very significant positive relationship with MDA and SP(r=0.94,0.89,P<0.01),some indicators showed a significant/very significant positive/negative correlation;The results of the principal component analysis showed that many physiological indicators such as SOD,POD,CAT,and LOX were closely related to fruit quality,and could be used as an important indicator to evaluate degree of oxidation of walnut with husk.The above results provided basic data for walnut with husk storage in folk freezer.

关 键 词：泡核桃 贮藏 生理指标 响应 

分 类 号：TS255.1[轻工技术与工程—农产品加工及贮藏工程]