复方扶芳藤合剂影响免疫抑制小鼠CD11b^(+)单核细胞CD69的表达  

Compound fufangteng mixture affects the expression of CD69 on CD11b^(+)monocytes in immunosuppressed mice

作　　者：丁健 陈果 李建明 李开奇 杨晨一 伍月榕 DING Jian;CHEN Guo;LI Jian-ming;LI Kai-qi;YANG Chen-yi;WU Yue-rong(Guangxi University of Traditional Chinese Medicine,Nanning 530200,China;Department of Spleen and Stomach Diseases,Hubei Provincial Hospital of Traditional Chinese Medicine,Wuhan 430060,China)

机构地区：[1]广西中医药大学、广西高发传染病中西医结合转化医学重点实验室、广西特色实验动物病证模型重点实验室,广西南宁530200 [2]湖北省中医院脾胃病科,湖北武汉430060

出　　处：《海南医学院学报》2023年第23期1774-1779,共6页Journal of Hainan Medical University

基　　金：国家自然科学基金项目(81860780);广西自然科学基金面上项目(2020GXNSFAA297162)。

摘　　要：目的:探讨复方扶芳藤合剂(compound fufangteng mixture,CFM)对免疫抑制小鼠脾及外周血CD11b^(+)单核细胞表面活化分子CD2、CD69及耗竭分子PD-1、CD95表达水平的影响。方法:将30只SPF级Balb/c雄性小鼠随机分为空白组(Control组)、免疫抑制模型组(CTX组)、复方扶芳藤合剂高、中、低剂量干预组(CTX+CFM-H组、CTX+CFM-M组、CTX+CFM-L组)、黄芪(astragalus particles,AP)干预组(CTX+AP组)。各组小鼠连续3 d腹腔注射环磷酰胺(CTX)70 mg/kg,遂按照分组方案分别给药10 d,末次给药24 h后取材。流式细胞术检测小鼠外周血CD11b^(+)单核细胞表面活化分子(CD2、CD69)和耗竭分子(PD-1、CD95)的表达。结果:与Control组相比,CTX+CFM-H组、CTX+CFM-M组、CTX+CFM-L组脾CD11b^(+)CD69表达水平均有改善趋势,并随着复方扶芳藤合剂剂量梯度降低。结论:复方扶芳藤合剂可调节免疫抑制小鼠脾CD11b^(+)细胞CD69的免疫功能。Objective:To investigate the effects of compound fufangteng mixture(CFM)on the expression levels of CD11b^(+)monocytes surface activation molecules CD2,CD69 and depleted molecules PD⁃1 and CD95 in spleen and peripheral blood of immunosuppressed mice.Methods:A total of 30 healthy SPF Balb/c male mice were randomly divided into the control group,the immunosuppressive model group(CTX group),the high,medium and low dose intervention group(CTX+CFM⁃H group,CTX+CFM⁃M group,CTX+CFM⁃L group),the Astragalus particles(AP)intervention group(CTX+AP group).The mice in each intervention group were injected intraperitoneally with cyclophosphamide(CTX)70 mg/kg for 3 consecutive days,and then administered for 10 days according to the group regimen,and the materials were taken 24 hours after the last dose.Flow cytometry detected the expression of CD11b^(+)monocytes surface activation molecules(CD2,CD69)and depleted molecules(PD⁃1,CD95)in mouse peripheral blood.Results:Compared with the control group,the expression levels of CD11b^(+)and CD69^(+)in the spleen of CTX+CFM⁃H,CTX+CFM⁃M and CTX+CFM⁃L groups showed an improvement trend,and de⁃creased with the dose gradient of CFM.Conclusion:CFM could regulate the immune function of splenic CD11b^(+)CD69 cells in immunosuppressed mice.

关 键 词：复方扶芳藤合剂 免疫抑制 CD69 CD95 

分 类 号：R285.5[医药卫生—中药学]