黄甲软肝颗粒解酒护肝药效学研究  

Pharmacodynamics of Huangjia Ruangan Granules for Alleviating Hangover and Protecting Liver

作　　者：张云 茅向军 张丽艳 于婷 唐海姣 刘洋 王宗权 华杰 ZHANG Yun;MAO Xiangjun;ZHANG Liyan;YU Ting;TANG Haijiao;LIU Yang;WANG Zongquan;HUA Jie(Guizhou University of Traditional Chinese Medicine,Guiyang 50025,China;Increase(Hengqin)Pharmaceutical Institute Company Limited,Zhuhai 519031,China;Zhejiang Wecome Pharmaceutical Company Limited,Lishui 323000,China)

机构地区：[1]贵州中医药大学,贵州贵阳550025 [2]盈科瑞(横琴)药物研究院有限公司,广东珠海519031 [3]浙江维康药业股份有限公司,浙江丽水323000

出　　处：《湖北民族大学学报（医学版）》2023年第4期1-6,共6页Journal of Hubei Minzu University(Medical Edition)

基　　金：广东省重点领域研发计划项目(2019B020201006);国家“重大新药创制”科技重大专项项目(2017ZX09301017)。

摘　　要：目的研究黄甲软肝颗粒对小鼠醉酒的解酒作用和CCl_(4)诱导小鼠急性肝损伤的护肝作用。方法取SPF级雄性昆明小鼠60只,按体质量随机分为正常组,模型组,低、中、高剂量黄甲软肝颗粒组,谷胱甘肽组,每组10只。除正常组与模型组灌服相同体积的蒸馏水外,其余各组给予相对应剂量的药物,正常组和模型组给予20 mL/kg蒸馏水,1次/d,连续7 d。末次给药前各组小鼠禁食12 h,末次给药60 min后,除正常组外的各组按14 mL/kg给予60°红星二锅头白酒灌胃。60 min后,取小鼠血液及肝脏组织,测定血中乙醇浓度,测定肝脏组织中谷胱甘肽、MDA含量及ADH活力,观察黄甲软肝颗粒解酒作用。另取SPF级雄性昆明小鼠60只,按体质量随机分为正常组,模型组,低、中、高剂量黄甲软肝颗粒组,阳性对照药谷胱甘肽组,每组10只,连续给药14 d,动物末次给药后禁食16 h,模型组及各给药组灌胃0.5%CCl_(4)植物油溶液5 mL/kg,正常组灌胃植物油5 mL/kg。24 h后,摘眼球取血及分离保存肝脏组织用于指标检测和组织病理学检查。观察黄甲软肝颗粒护肝作用。结果与模型组比较,黄甲软肝颗粒高剂量组和谷胱甘肽组小鼠血液中乙醇浓度显著降低(P<0.05)。与模型组比较,黄甲软肝颗粒高剂量组谷胱甘肽组的谷胱甘肽含量显著增加(P<0.05);黄甲软肝颗粒中剂量组和谷胱甘肽组MDA含量显著降低(P<0.05);黄甲软肝颗粒高剂量组和谷胱甘肽组ADH活性显著增加(P<0.05)。与模型组比较,黄甲软肝颗粒中剂量组和谷胱甘肽组血清ALT、AST活性均显著降低(P<0.05或P<0.01);黄甲软肝颗粒中剂量组和谷胱甘肽组肝脏组织MDA含量显著降低(P<0.05)。谷胱甘肽组的谷胱甘肽含量显著增加(P<0.05)。与模型组比较,黄甲软肝颗粒中、高剂量组和谷胱甘肽组小鼠肝细胞结构有一定改善,细胞浆和细胞核溶解程度有所减轻。结论黄甲软肝颗粒能降低急性酒精性�Objective To study the antialcoholic effect of Huangjia Ruangan Granules on mice intoxication and the hepatoprotective effect of CCl_(4)-induced acute liver injury in mice.Methods 60 SPF grade male Kunming mice were randomly divided into normal control group,model control group,low,medium and high dose Huangjia Ruangan Granules group and positive control glutathione group,with 10 mice in each group.Except for the normal control group and the model control group,which were infused with the same volume of distilled water,the remaining groups were given the corresponding doses of drugs.The normal group and model group were given 20 mL/kg distilled water once a day for 7 consecutive days.Before the last administration,the mice in each group were fasted for 12 h,and after the last administration for 60 min,all groups except the normal group were given 60°red star Erguotou white wine at the rate of 14 mL/kg.After 60 min,the blood and liver tissues of mice were taken to determine the concentration of alcohol in blood,the content of glutathione and MDA in liver tissues and the activity of ADH,and the antialcohol effect of Huangjia Ruangan Granules was observed.60 SPF grade male Kunming mice were randomly divided into normal control group,model control group,low,medium and high dose Huangjia Ruagan Granules group and positive control glutathione group with 10 mice in each group according to body weight.The drug was administered continuously for 14 d.After the last administration,fasting was performed for 16 h.The model group and the drug administration groups were gavaged with 0.5%CCl_(4)vegetable oil solution,5 mL/kg,and the normal group was gavaged with 5 mL/kg of vegetable oil.24 h later,blood was collected from the eyeballs and liver tissue was separated and preserved for the index test and histopathological examination.The protective effect of Huangjia Ruangan Granules on liver was observed.Results Compared with alcohol acute liver injury model group,the concentration of alcohol in blood of mice in high dose Huangji

关 键 词：黄甲软肝颗粒 解酒护肝 药效学 

分 类 号：R285.5[医药卫生—中药学]