超高效液相色谱-串联质谱法测定重感灵片中多种成分的含量  

UPLC-MS/MS for the Determination of Multiple Ingredients in ZhongGanLing Tablets

作　　者：沈麟 SHEN Lin(Quanzhou Institue for Food and Drug Control,Quanzhou 362000,China)

机构地区：[1]泉州市食品药品检验所,福建泉州362000

出　　处：《海峡药学》2023年第11期40-43,共4页Strait Pharmaceutical Journal

摘　　要：目的建立超高效液相色谱-串联质谱法同时测定重感灵片中多种成分含量的一种方法。方法用Waters C 18 ACQUITY UPLC BEH柱,0.1%甲酸水溶液(含10 mmol·L^(-1)乙酸铵)为A相、乙腈为B相进行UPLC梯度洗脱,流速为0.3 mL·min^(-1),进样量为1μL,采用正负离子自动切换模式进行多反应监测。结果安乃近、马来酸氯苯那敏、葛根素、青蒿素的线性范围分别为1058~10580 ng·mL^(-1)、10.73~107.3 ng·mL^(-1)、129.0~1290 ng·mL^(-1)、4.364~43.64 ng·mL^(-1)(r 2=0.9962、0.9986、0.9996、0.9938),平均回收率为99.4%、98.8%、97.0%、97.6%,RSD为1.4%、1.9%、2.5%、1.2%(n=6)。结论该方法简捷快速有效,实现了多组分同时测定。OBJECTIVE To establish a method for the simultaneous determination of multiple ingredients in ZhongGanLing Tablets by UPLC-MS/MS.METHODS With the Waters C 18 ACQUITY UPLC BEH column,0.1%formic acid in water(containing 10 mmol·L^(-1) ammonium acetate)as mobile phase A and acetonitrile as mobile phase B for UPLC gradient elution,with the flow rate of 0.3 mL·min^(-1) and the injection volume was 1μL,multiple reaction monitoring was carried out by auto switch mode of positive and negative ions.RESULTS The linear ranges of metamizole sodium,chlorphenamine maleate,puerarin and artemisinin were 1058-10580 ng·mL^(-1),10.73-107.3 ng·mL^(-1),129.0-1290 ng·mL^(-1),4.364-43.64 ng·mL^(-1)(r 2=0.9962、0.9986、0.9996、0.9938),the average recovery rates were 99.4%,98.8%,97.0%,97.6%,and RSD was 1.4%,1.9%,2.5%,1.2%(n=6)。CONCLUSION This method was uncomplicated,rapid and efficacious,and accomplished the simultaneous determination of multiple ingredients.

关 键 词：超高效液相色谱-串联质谱法 重感灵片 安乃近 马来酸氯苯那敏 葛根素 青蒿素 

分 类 号：R927[医药卫生—药学]