多房棘球蚴原头节感染后阻断PD-1对巨噬细胞吞噬功能的影响  

Effects of PD-1 blockade on macrophage phagocytosis after Echinococcus multilocularis protoscoleces infection

作　　者：张涛[1,2,3] 张耀刚[1] 杨紫晗 沈银红 马艳艳 ZHANG Tao;ZHANG Yao-gang;YANG Zi-han;SHEN Yin-hong;MA Yan-yan(Qinghai University Affiliated Hospital,Xining 810000,China;Research Center for High Altitude Medicine,Xining 810001,China;Laboratory for High Altitude Medicine of Qinghai Province,Xining 810000,China)

机构地区：[1]青海大学附属医院,西宁810000 [2]青海大学高原医学研究中心,西宁810000 [3]青海省高原医学实验室,西宁810000

出　　处：《中国人兽共患病学报》2023年第11期1095-1099,共5页Chinese Journal of Zoonoses

基　　金：国家自然科学基金(No.82360128);青海省科技厅项目(No.2021-SF-135);青海大学附属医院中青年科研基金一般项目(No.ASRF-2021-YB-02);青海省“昆仑英才·高端创新创业人才”联合资助。

摘　　要：目的研究多房棘球蚴原头节感染后巨噬细胞吞噬功能的变化。方法免疫组化分析巨噬细胞在泡型包虫病患者肝脏中的分布;阻断PD-1、多房棘球蚴原头节与巨噬细胞共培养分析巨噬细胞的吞噬功能和CD47、PD-1的表达水平。结果CD68标记巨噬细胞发现巨噬细胞在泡型包虫病患者病灶近旁肝脏组织聚集;多房棘球蚴原头节感染后与对照组相比,大肠杆菌的荧光强度降低(P<0.001),CD47和PD-1的表达增加;多房棘球蚴原头节感染给予信迪利单抗阻断PD-1与单独多房棘球蚴感染组相比,大肠杆菌的荧光强度增加(q=40.63,P<0.001)。结论阻断PD-1可恢复多房棘球蚴原头节感染巨噬细胞的吞噬功能。This study analyzed the changes in macrophage phagocytosis after Echinococcus multilocularis protoscoleces(PSC)infection.The distribution of macrophages in hepatic alveolar echinococcosis patients was analyzed by immunohistochemistry.PD-1 blockade,PSCs and macrophages were co-cultured to analyze macrophage phagocytosis and the expression levels of CD47 and PD-1.Macrophages labeled by CD68 aggregated in close live tissue from the lession of alveolar echinococcosis patients.PSC infection resulted in lower intensity of Escherichia coli and higher expression of CD47 and PD-1 than observed in the control group(P<0.001).The intensity of Escherichia coli increasedin the PD-1 blockade group after PSC infection(q=40.63,P<0.05).PD-1 blockade restored macrophage phagocytosis after PSC infection.

关 键 词：多房棘球蚴原头节 程序性死亡分子1 巨噬细胞 吞噬功能 

分 类 号：R383.3[医药卫生—医学寄生虫学]