The plasmodesmata-associated β-1,3-glucanase gene GhPdBG regulates fiber development in cotton  

作　　者：Yijie Fan Shuangshuang Lin Yanhui Lyu Haihong Shang Youlu Yuan Zhengmin Tang Chengzhi Jiao Aiyun Chen Piyi Xing Li Zhang Yuxiao Sun Haixia Guo Tongtong Li Zhonghai Ren Fanchang Zeng 

机构地区：[1]State Key Laboratory of Crop Biology,College of Agronomy,Shandong Agricultural University,Tai’an 271018,Shandong,China [2]State Key Laboratory of Cotton Biology,Key Laboratory of Biological and Genetic Breeding of Cotton,The Ministry of Agriculture,Institute of Cotton Research,Chinese Academy of Agricultural Sciences,Anyang 455000,Henan,China [3]Zhengzhou Research Base,State Key Laboratory of Cotton Biology,Zhengzhou University,Zhengzhou 450001,Henan,China [4]State Key Laboratory of Crop Biology,College of Horticulture Science and Engineering,Shandong Agricultural University,Tai’an 271018,Shandong,China

出　　处：《The Crop Journal》2023年第6期1665-1674,共10页作物学报（英文版）

基　　金：the State Key Laboratory of Cotton Biology Open Fund(CB2021A04);the Agricultural Seed Project of Shandong Province(2020LZGC002);the Science Foundation of Shandong Province(ZR2020MC107)。

摘　　要：Trichomes are specialized structures that originate from epidermal cells of organs in higher plants.The cotton fiber is a unique single-celled trichome that elongates from the seed coat epidermis.Cotton(Gossypium hirsutum)fibers and trichomes are models for cell differentiation.In an attempt to elucidate the intercellular factors that regulate fiber and trichome cell development,we identified a plasmodesmal β-1,3-glucanase gene(designated GhPdBG)controlling the opening and closing of plasmodesmata in cotton fibers.Structural and evolutionary analysis showed haplotypic variation in the promoter region of the GhPdBG gene among 352 cotton accessions,but high conservation in the coding region.GhPdBG was expressed predominantly in cotton fibers and localized to plasmodesmata(PD).Expression patterns of PdBG that corresponded to PD permeability were apparent during fiber development in G.hirsutum and G.barbadense.The PdBG-mediated opening-closure of PD appears to be involved in fiber development and may account for the contrasting fiber traits of these two species.Ectopic expression of GhPdBG revealed that it functions in regulating fiber and trichome length and/or density by modulating plasmodesmatal permeability.This finding suggests that plasmodesmal targeting of GhPdBG,as a switch of intercellular channels,regulates single-celled fiber and trichome development in cotton.

关 键 词：Fiber/trichome Β-1 3-GLUCANASE Functional analysis Evolutional variation 

分 类 号：S562[农业科学—作物学]