Ectopic expression of OsNF-YA8,an endosperm-specific nuclear factor Y transcription-factor gene,causes vegetative and reproductive development defects in rice  

作　　者：Baixiao Niu Jing Xu Zhiguo E Zhenyu Zhang Xinming Lu Chen Chen 

机构地区：[1]Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Zhongshan Biological Breeding Laboratory,Agricultural College of Yangzhou University,Yangzhou 225009,Jiangsu,China [2]Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops,Yangzhou University,Yangzhou 225009,Jiangsu,China [3]Key Laboratory of Plant Functional Genomics of the Ministry of Education,Agricultural College of Yangzhou University,Yangzhou 225009,Jiangsu,China [4]Key Laboratory of Rice Biology,China National Rice Research Institute,Hangzhou 311400,Zhejiang,China

出　　处：《The Crop Journal》2023年第6期1719-1730,共12页作物学报（英文版）

基　　金：the National Natural Science Foundation of China(31701392 and 32170344);the Six Talent Peaks Project in Jiangsu Province(NY-142);the Jiangsu Province Government(JBGS[2021]001);the Independent Scientific Research Project Funds of the Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding(PLR202101).

摘　　要：Nuclear factor Y(NF-Y),a group of conserved transcription-factor complexes that consist of NF-YA,B,and C subunits,is essential for developmental regulation and for responses to environmental changes in eukaryotes.We previously found that some NF-Y genes,such as OsNF-YA8,were expressed specifically in the endosperm of rice.In the present study,overexpression of OsNF-YA8 in rice resulted in reduced plant height due to suppressed cell elongation in internodes.Gibberellin(GA)biosynthetic genes,including OsCPS1,OsGA20ox1,and OsGA20ox2,were down-regulated.OsNF-YA8 bound to the promoters of these genes to repress their expression.Endogenous GA content was decreased in OsNF-YA8 overexpressors,whose dwarf phenotype could be partially rescued by exogenous GA treatment.The findings suggested that ectopic expression of OsNF-YA8 causes defective GA biosynthesis in vegetative stage.Heading date in OsNF-YA8 overexpressors was delayed,especially under short-day conditions.OsNFYA8 bound to the promoter of Heading Date 3a(Hd3a),the florigen gene in rice,to negatively regulate flowering.Either ectopic activation or knockout of OsNF-YA8 impaired seed development,as indicated by reduced seed size and increased grain chalkiness.These results suggest that ectopic expression of the endosperm-specific OsNF-YA8 in rice disrupts both vegetative and reproductive development.

关 键 词：RICE OsNF-YA8 Plant height Flowering time Seed development 

分 类 号：S311[农业科学—作物栽培与耕作技术]