GA Associated Dwarf 5 encodes an ent-kaurenoic acid oxidase required for maize gibberellin biosynthesis and morphogenesis  

作　　者：Zuliang Li Baozhu Li Junli Zhang Hongliang Wang Mao Wang Siyi Guo Pengtao Wang Zhi Li David WGalbraith Dandan Li Chun-Peng Song 

机构地区：[1]State Key Laboratory of Crop Stress Adaptation and Improvement,State Key Laboratory of Cotton Biology,School of Life Sciences,Henan University,Kaifeng 475004,Henan,China [2]Sanya Institute of Henan University,Sanya 572025,Hainan,China [3]Academy for Advanced Interdisciplinary Studies,Henan University,Kaifeng 475004,Henan,China [4]Shanghai Center for Plant Stress Biology,CAS Center for Excellence in Molecular Plant Sciences,Chinese Academy of Sciences,Shanghai 200032,China [5]National Engineering Research Centre for Wheat,State Key Laboratory of Wheat and Maize Crop Science,College of Agronomy,Henan Agricultural University,Zhengzhou 450046,Henan,China [6]School of Plant Sciences and Bio5 Institute,The University of Arizona,Tucson,AZ 85721,USA [7]China-UK-NYNU-RRes Joint Laboratory of Insect Biology,Henan Key Laboratory of Insect Biology in Funiu Mountain,Nanyang Normal University,Nanyang 473061,Henan,China

出　　处：《The Crop Journal》2023年第6期1742-1751,共10页作物学报（英文版）

基　　金：the National Natural Science Foundation of China(U21A20206,Chun-Peng Song);the Project of Sanya Yazhou Bay Science and Technology City(SCKJJYRC-2022-78,Baozhu Li);the Program for Innovative Research Team(in Science and Technology)in University of Henan Province(21IRTSTHN019,Siyi Guo);the 111 Project of China(D16014).

摘　　要：Gibberellin(GA)functions in plant growth and development.However,genes involved in the biosynthesis and regulation of GA in crop plants are poorly understood.We isolated the mutant gad5-1(GAAssociated Dwarf 5),characterized by dwarfing,short internodes,and dark green and short leaves.Map-based gene cloning and allelic verification confirmed that ZmGAD5 encodes ent-kaurenoic acid oxidase(KAO),which catalyzes KA(ent-kaurenoic acid)to GA12 conversion during GA biosynthesis in maize.ZmGAD5 is localized to the endoplasmic reticulum and is present in multiple maize organs.In gad5-1,the expression of ZmGAD5 is severely reduced,and the levels of the direct substrate of KAO,KA,is increased,leading to a reduction in GA content.The abnormal phenotype of gad5-1 was restored by exogenous application of GA3.The biomass,plant height,and levels of GA12 and GA3 in transgenic Arabidopsis overexpressing ZmGAD5 were increased in comparison with the corresponding controls Col-0.These findings deepen our understanding of genes involved in GA biosynthesis,and could lead to the development of maize lines with improved architecture and higher planting-density tolerance.

关 键 词：Maize(Zea mays L.) ZmGAD5 GA biosynthesis KAO 

分 类 号：S513[农业科学—作物学]