Haplotype variation and KASP markers for SiPSY1-A key gene controlling yellow kernel pigmentation in foxtail millet  

作　　者：Rongjun Zuo Yanyan Zhang Yanbing Yang Chunfang Wang Hui Zhi Linlin Zhang Sha Tang Yanan Guan Shunguo Li Ruhong Cheng Zhonglin Shang Guanqing Jia Xianmin Diao 

机构地区：[1]Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081,China [2]Crop Research Institute,Shandong Academy of Agricultural Sciences,Jinan 250100,Shandong,China [3]Institute of Millet Crops,Hebei Academy of Agricultural and Forestry Sciences,Shijiazhuang 050035,Hebei,China [4]College of Life Sciences,Hebei Normal University,Shijiazhuang 050024,Hebei,China

出　　处：《The Crop Journal》2023年第6期1902-1911,共10页作物学报（英文版）

基　　金：the National Natural Science Foundation of China(U21A20216);the National Key Research and Development Program of China(2018YFD1000705/2018YFD1000700);Fundamental Research Funds of the Chinese Academy of Agricultural Sciences(Grant to Guanqing Jia,1610092016116,Y2017JC15);China Agricultural Research System(CARS06-14.5-A04);State Key Laboratory of Crop Gene Resources and Breeding,Key Laboratory of Grain Crop Genetic Resources Evaluation and Utilization,Key Laboratory of Crop Gene Resource and Germplasm Enhancement(MOA),and Technology Innovation Program of the Chinese Academy of Agricultural Sciences.

摘　　要：Carotenoid biosynthesis and accumulation are important in determining nutritional and commercial value of crop products.Yellow pigmentation of mature kernels caused by carotenoids is considered a vital quality trait in foxtail millet,an ancient and widely cultivated cereal crop across the world.Genomic regions associated with yellow pigment content(YPC),lutein and zeaxanthin in foxtail millet grains were identified by genome-wide association analysis(GWAS),and SiPSY1(Phytoene synthase 1 which regulates formation of the 40-carbon backbone of carotenoids)was confirmed as the main contributor to all three components by knockout and overexpression analysis.SiPSY1 was expressed in seedlings,leaves,panicles,and mature seeds,and was subcellularly localized to chloroplasts.Transcription of SiPSY1 in 15 DAP immature grains was responsible for YPC in mature seeds.Selection of SiPSY1 combined with increased YPC in mature grains during domestication of foxtail millet was confirmed.Haplotype analysis suggested that expression level of SiPSY1 could be a selection target for future breeding programs,and a KASP marker was developed for selection of favorable SiPSY1 alleles in breeding.The results of this work will benefit nutritional and commercial improvement of foxtail millet varieties,as well as other cereal crops.

关 键 词：Carotenoids LUTEIN ZEAXANTHIN Marker assisted selection Setaria italica 

分 类 号：S515[农业科学—作物学]