不同碳氮比对生物絮凝技术(BFT)养殖水体中土腥味物质积累量的影响  

Effects of different carbon to nitrogen ratios on off flavor in BFT

作　　者：崔佳勤 章海鑫[1,2] 罗国芝[1,3,4] 谭洪新 CUI Jiaqin;ZHANG Haixin;LUO Guozhi;TAN Hongxin(Shanghai Engineering Research Center of Aquaculture,Shanghai Ocean University,Shanghai 201306,China;Jiangxi Fisheries Research Institute,Nanchang 330039,Jiangxi,China;Shanghai Collaborative Innovation Center for Cultivating Elite Breeds and Green-culture of Aquaculture Animals,Shanghai 201306,China;Key Laboratory of Freshwater Aquatic Germplasm Resources,Ministry of Agriculture and Rural Affairs,Shanghai Ocean University,Shanghai 201306,China)

机构地区：[1]上海海洋大学上海水产养殖工程技术研究中心,上海201306 [2]江西省水产科学研究所,江西南昌330039 [3]上海市水产动物良种创制与绿色养殖协同创新中心,上海201306 [4]上海海洋大学水产科学国家级实验教学示范中心,上海201306

出　　处：《渔业现代化》2023年第6期9-16,共8页Fishery Modernization

基　　金：上海市科学技术委员地方院校能力提升项目“凡纳滨对虾陆基循环水高效养殖工艺研究与示范(23010502300)”。

摘　　要：为探究生物絮凝技术(Biofloc technology,BFT)中不同碳氮比对水体中土腥味物质积累量的影响,以养虾排放的生物絮团为研究对象,设置了不同的初始溶解性有机碳(Dissolved Organic Carbon,DOC)/溶解性无机氮(Dissolved Inorganic Nitrogen,DIN)梯度,分别为A组(不添加葡萄糖组,C/N为6)、B组(C/N为10)和C组(C/N为20),进行为期7 d的试验,每日进行一次水质监测,试验开始和结束时检测BFT水体中土臭素(Geosmin,GSM)和二甲基异莰醇(2-Methylisoborneol,2-MIB)的含量,取生物絮团进行营养指标检测。结果显示:B组的GSM和2-MIB积累量分别为22.41 ng/L和-0.19 ng/L,显著低于A组(76.4 ng/L和4.43 ng/L)和C组(50.98 ng/L和3.52 ng/L,P<0.05);试验期间,各组水体中总氨氮和亚硝酸盐氮质量浓度均处于较低水平,分别低于0.10 mg/L和0.15 mg/L;B组生物絮团的粗蛋白含量最高,为31.64%±0.22%。研究表明,添加葡萄糖能有效减少GSM和2-MIB的生成积累量,调控BFT水体中的C/N为10时,减少GSM和2-MIB生成积累量的效果最明显,同时有水质改良效果。To investigate the effect of different C/N on the accumulation of off flavor in biofloc reactor,different initial DOC/DIN(Dissolved organic carbon,DOC:Dissolved inorganic nitrogen,DIN)gradients were set up for group A(no glucose added,C/N of 6),group B(C/N of 10)and group C(C/N of 20)by adding glucose to the bioflocs released from shrimp farming and a 7-day experiment was conducted.Water quality monitoring was carried out everyday.The concentrations of Geosmin(GSM)and 2-Methylisoborneol(2-MIB)in the BFT water were detected at the beginning and end of the experiment and the flocs were taken for nutrient index detection.The results showed that the accumulation of Geosmin(GSM)and 2-Methylisoborneol(2-MIB)was 22.41 ng/L and-0.19 ng/L at C/N of 10,which were significantly lower than those in the C/N of 6 group(76.4 ng/L and 4.43 ng/L)and C/N of 20 group(50.98 ng/L and 3.52 ng/L),respectively;the total ammonia nitrogen and nitrite nitrogen concentrations were lower than 0.10 mg/L and 0.15 mg/L,respectively,in all groups during the experiment;the crude protein content of bioflocs was highest at 31.64%±0.22%at C/N of 10.It was shown that the addition of glucose can effectively reduce the accumulation of GSM and 2-MIB production,and the most effective of reducing the accumulation of GSM and 2-MIB production was achieved when the C/N in the biofloc reactor was 10,as well as having water quality improvement effect.

关 键 词：生物絮凝技术 碳氮比 土臭素 二甲基异莰醇 循环水养殖 

分 类 号：S964.9[农业科学—水产养殖]