2株金黄色葡萄球菌耐药性分析及中药对其部分耐药基因表达的影响  

Drug Resistance Analysis of 2 Staphylococcus aureus Strains and the Influence of Traditional Chinese Medicine on Expression of Some Drug Resistance Genes

作　　者：张亚倩 王亚男 郭上朝 张洪伟 史秋梅[1] 蒋海龙 赵希艳 刘永波 高光平[1] ZHANG Yaqian;WANG Yanan;GUO Shangzhao;ZHANG Hongwei;SHI Qiumei;JIANG Hailong;ZHAO Xiyan;LIU Yongbo;GAO Guangping(Hebei Key Laboratory of Preventive Veterinary Medicine,College of Animal Science and Technology,Hebei Normal University of Science&Technology,Qinhuangdao 066004,China;Chengde Academy of Agricultural and Forestry Sciences,Chengde 067000,China)

机构地区：[1]河北科技师范学院动物科技学院,河北省预防兽医学重点实验室,秦皇岛066004 [2]承德市农林科学院,承德067000

出　　处：《中国畜牧兽医》2023年第12期5204-5212,共9页China Animal Husbandry & Veterinary Medicine

基　　金：河北科技师范学院科学研究基金资助(2021YB001);河北省现代农业产业技术体系肉牛创新团队(HBCT2023190201);河北省重点研发计划项目(22326612D);河北省奶肉牛呼吸道疾病综合征诊断与防控技术研究(236Z6604G)。

摘　　要：【目的】研究确定金黄色葡萄球菌对常见抗菌药物及中药的耐药程度,并探究中药对金黄色葡萄球菌耐药基因表达的影响。【方法】采用纸片扩散法检测2株金黄色葡萄球菌SA1、SA2菌株对常用抗菌药的耐药性,并选取常见10味中药对其进行最小抑菌浓度(MIC)测定,筛选出4味效果最好的中药。菌株分别加入至不同浓度4味中药(0、1/2、1/4、1/8 MIC)的含3%葡萄糖BHI培养基,通过实时荧光定量PCR检测亚抑菌浓度下4味中药对金黄色葡萄球菌部分耐药基因表达量的影响。【结果】药敏试验结果显示,SA1菌株对氨苄西林高度耐药,对头孢他啶中度耐药,对其余药物敏感;SA2菌株对氨苄西林、青霉素、红霉素、克林霉素、万古霉素、苯唑西林耐药性较高,对其余药物敏感。10味中药水煎液对2株金黄色葡萄球菌MIC测定结果显示,黄连、黄柏、五倍子和地榆对2株菌株的抑制效果最佳。实时荧光定量PCR结果显示,不同药液浓度下,五倍子极显著降低SA1菌株mecA、mecB基因表达量(P<0.01);黄连和黄柏极显著降低SA1菌株mecB基因表达量(P<0.01);黄连和地榆极显著降低SA2菌株mecA基因表达量(P<0.01)。【结论】中药对金黄色葡萄球菌具有抑菌效果,并在一定程度上降低相关耐药基因的表达水平,其中黄连、五倍子和地榆效果最佳,黄柏次之。研究结果为中药对金黄色葡萄球菌的防治提供了参考依据。【Objective】The purpose of this experiment was to study and determine the degree of resistance of Staphylococcus aureus to common antibiotics and traditional Chinese medicine,and to explore the influence of traditional Chinese medicine on the expression of resistance genes in Staphylococcus aureus.【Method】The resistance of Staphylococcus aureus SA1 and SA2 to commonly used antibiotics was detected by Kirby-Bauer method.The minimum inhibitory concentrations(MIC)of 10 traditional Chinese medicines was determined,and the 4 traditional Chinese medicines with the best effect were selected.The strains were added to BHI medium containing 3%glucose at different concentrations of 4 traditional Chinese medicine(0,1/2,1/4 and 1/8 MIC),and the effects of 4 traditional Chinese medicines on the expression of some drug resistance genes in Staphylococcus aureus were detected by Real-time quantitative PCR.【Result】The results of drug sensitivity test showed that SA1 strain was highly resistant to ampicillin,moderately resistant to ceftazidime,and sensitive to other drugs.SA2 strain had high resistance to ampicillin,penicillin,erythromycin,clindamycin,vancomycin and oxacillin,and sensitive to other drugs.The results of MIC determination of 2 strains of Staphylococcus aureus by 10 kinds of traditional Chinese medicine decoction showed that Coptis chinensis,Cortex Phellodendri chinensis,Galla chinensis and Sanguisorba officinalis L.had the best inhibitory effect on 2 strains.Real-time quantitative PCR results showed,under different liquid concentrations,Galla chinensis significantly decreased the expression of mecA and mecB genes in SA1 strain(P<0.01).Coptis chinensis and Cortex Phellodendri chinensis significantly decreased mecB gene expression in SA1 strain(P<0.01).Coptis chinensis and Sanguisorba officinalis L.significantly decreased mecA gene expression in SA2 strain(P<0.01).【Conclusion】Traditional Chinese medicine could inhibit Staphylococcus aureus and reduce the expression level of related drug resistance genes

关 键 词：金黄色葡萄球菌 耐药性 中药 耐药基因 

分 类 号：S859.3[农业科学—临床兽医学]