早期缺氧对小鼠心肌细胞miR-34a表达及凋亡的影响  

Effect of early hypoxia on the expression and apoptosis of miR-34a in mouse cardiomyocytes

作　　者：刘龙梅 王仲朝 石懿玉 韩超 许慧玉 安健 韩学斌 LIU Longmei;WANG Zhongchao;SHI Yiyu;HAN Chao;XU Huiyu;AN Jian;HAN Xuebin(Laboratory,Shanxi Cardiovascular Hospital,Shanxi Province,Taiyuan030024,China;Department of Cardiology,Shanxi Cardiovascular Hospital,Shanxi Province,Taiyuan030024,China)

机构地区：[1]山西省心血管病医院实验室,山西太原030024 [2]山西省心血管病医院心内科,山西太原030024

出　　处：《中国当代医药》2023年第34期14-17,共4页China Modern Medicine

基　　金：山西省重点研发计划(国际科技合作)项目(201803D421052);山西省心血管病医院科研激励计划项目(XYS 20170204)。

摘　　要：目的 探讨早期缺氧对小鼠心肌细胞miR-34a表达及凋亡的影响。方法 原代培养小鼠乳鼠心肌细胞并培养,缺氧6 h造模,使用anti-miR-34a纳米颗粒干预。将其随机分为三组:对照组(C组)、缺氧组(Ⅰ组)和缺氧+anti-miR-34a纳米颗粒组(Ⅰ+NP组)。采用qRT-PCR检测miR-34a的表达,Western blot检测乙醛脱氢酶2(ALDH2)、caspase 3的表达。结果 Ⅰ组的miR-34a表达、caspase 3表达均高于C组,差异均有统计学意义(P<0.05);Ⅰ组的ALDH2表达低于C组,差异有统计学意义(P<0.05);Ⅰ+NP组的miR-34a表达、caspase 3表达均低于Ⅰ组,差异均有统计学意义(P<0.05);Ⅰ+NP组的ALDH2水平高于Ⅰ组,差异有统计学意义(P<0.05)。结论 早期缺氧上调小鼠心肌细胞miR-34a表达,下调下游ALDH2蛋白的表达;Anti-miR-34a纳米颗粒可以进入心肌细胞缓解早期缺氧引起的凋亡。Objective To explore the effect of early hypoxia on the expression and apoptosis of miR-34a in mouse cardiomyocytes.Methods Primary culture of neonatal mouse cardiomyocytes in 6 h hypoxia was used to establish a model,intervening with anti-miR-34a nanoparticles.They were randomly divided into three groups:control group(group C),ischemia group(group I),and ischemia+anti miR-34a nanoparticle group(group I+NP).The expression of miR-34a was measured using real-time quantitative PCR(qRT-PCR),and the expression of acetaldehyde dehydrogenase 2(ALDH2)and caspase 3 was detected by Western blot.Results The expression of miR-34a and caspase 3 in group I were higher than those in group C,with statistically significant differences(P<0.05).The expression of ALDH2 in group I was lower than that in group C,with a statistically significant difference(P<0.05).The expression levels of miR-34a and caspase 3 in the group I+NP were lower than those in the group I,and the differences were statistically significant(P<0.05).The ALDH2 level in the group I+NP was higher than that in the group I,with a statistically significant difference(P<0.05).Conclusion Early hypoxia upregulates the expression of miR-34a in mouse cardiomyocytes and downregulates the expression of downstream ALDH2 protein.Anti-miR-34a nanoparticles can enter myocardial cells to alleviate apoptosis caused by early hypoxia.

关 键 词：MIR-34A 缺氧 纳米颗粒 心肌细胞凋亡 

分 类 号：R319[医药卫生—基础医学]