核桃CDPK基因家族鉴定与转录表达分析  

Identification and transcriptional expression analysis of CDPK gene family in Juglans regia L.

作　　者：庄丽丽[1] 宋林眉 王芳 李玉群[1] 张柱岐[1] 刘凯 ZHUANG Lili;SONG Linmei;WANG Fang;LI Yuqun;ZHANG Zhuqi;LIU Kai(Binzhou Polytechnic,Binzhou,Shandong 256603,China;Qingtian Sub District Office of Binzhou High Tech Zone,Binzhou,Shandong 256603,China)

机构地区：[1]滨州职业学院,山东滨州256603 [2]滨州高新区青田街道办事处,山东滨州256603

出　　处：《落叶果树》2023年第6期32-35,共4页Deciduous Fruits

基　　金：滨州职业学院博士基金(2022bzbs01);“十四五”国家重点研发计划课题(2022YFD2200402);河北省重点研发计划项目(21326352D)。

摘　　要：钙依赖型蛋白激酶(CDPK)在植物响应逆境胁迫过程中起着重要作用,本研究在核桃全基因组数据库中利用隐马尔可夫模型鉴定CDPK家族成员基因,对该家族成员进行了结构分析,并对冷胁迫和不同组织的转录水平进行分析。结果显示,核桃CDPK基因家族包含38个成员,开放阅读框为399~2181 bp,蛋白大小为132~645个氨基酸,等电点为5.34~9.14,亚细胞定位预测该38个基因均定位于细胞核。在清香核桃冷胁迫不同时间后进行转录表达分析,发现JrCDPK4呈现显著上升趋势,并在根和嫩叶中表达较高,推测该基因可能参与响应冷胁迫以及根、嫩叶的生长发育,本研究结果为进一步研究该基因响应冷胁迫的机理奠定了基础。Calcium-dependent protein kinase(CDPK)plays an important role in plant response to stress.In this study,CDPK family members were identified using Hidden Markov Model(HMM)in the whole genome database of walnut.The structure of CDPK family members was analyzed,and the transcription levels of CDPK family members were analyzed under cold stress and in different tissues.The results showed that the CDPK gene family contained 38 members,which were located on 14 chromosomes,with open reading frames ranging from 399 to 2181 bp,protein sizes ranging from 132 to 645 amino acids,and isoelectric points ranging from 5.34 to 9.14.Subcellular localization predicted that all 38 members were located in the nucleus.Transcriptional expression analysis of fresh walnut after different time of cold stress revealed that JrCDPK4 showed a significant increasing trend and was highly expressed in roots and young leaves,suggesting that this gene might be involved in the response to cold stress and in the growth and development of roots and young leaves.The results of this study laid a foundation for further research on the mechanism of this gene’s response to cold stress.

关 键 词：核桃 冷胁迫 CDPK家族 表达分析 

分 类 号：S664.1[农业科学—果树学]