臭常山内生真菌绿色合成纳米银的优化、表征及催化活性研究  

Optimization,characterization,and catalytic activity of green synthesis of silver nanoparticles by endophytic fungus from Orixa japonica

作　　者：苟琴 张振 GOU Qin;ZHANG Zhen(School of Liquor and Food Engineering,Guizhou University,Guiyang 550000,China;Guizhou Academy of Testing and Analysis,Guiyang 550000,China)

机构地区：[1]贵州大学酿酒与食品工程学院,贵州贵阳550000 [2]贵州省分析测试研究院,贵州贵阳550000

出　　处：《食品与发酵工业》2023年第23期149-155,共7页Food and Fermentation Industries

基　　金：贵州科学院博士科研启动基金项目(黔科院R字[2022]01号)。

摘　　要：为探究臭常山内生真菌Diaporthe orixae sp.nov.用于生物合成纳米银及其催化活性的研究,利用臭常山内生真菌D.orixae菌丝裂解液合成纳米银,对其合成条件进行优化调控,将合成的纳米银进行表征,并进一步考察了合成的纳米银的催化活性。反应时间12 h、菌株生物量5 g、AgNO_(3)浓度2.0 mmol/L、pH 8、温度55℃为菌株D.orixae菌丝裂解液合成纳米银的最优条件;扫描电镜显示合成的纳米银为球状,分散性较好;X射线衍射图谱显示,合成的纳米银晶体结构为面心立方结构;通过傅里叶变换红外光谱FT-IR分析可得菌株D.orixae自身携带的羟基、羰基、酰胺键等官能团可能有助于纳米银的形成和稳定。此外,该实验合成的纳米银对4-硝基苯酚、甲基橙和亚甲基蓝都有较好的催化活性。臭常山内生真菌D.orixae菌丝裂解液可用于绿色合成纳米银,合成的纳米银在绿色催化降解4-硝基苯酚和有机染料领域具有潜在的研究价值。In order to investigate the usage of endophytic fungus Diaporthe orixae sp.nov.from the Orixa japonica on the biosynthesis of silver nanoparticles and their catalytic activity,the endophytic fungus D.orixae was used in this study,the silver nanoparticles were synthesized by the hypha lysate,the synthesis conditions were optimized,the synthesized silver nanoparticles were characterized,and the catalytic activity of the synthesized silver nanoparticles was further investigated.The optimum conditions for the synthesis of silver nanoparticles in the hypha lysate of strain D.orixae were as follows:reaction time 12 h,strain biomass 5 g,AgNO_(3) concentration 2.0 mmol/L,pH 8,and 55℃.Scanning electron microscope results showed that the synthesized silver nanoparticles were spherical and had good dispersion;the X-ray diffraction pattern showed that the synthesized nano-silver crystal structure was face-centered cubic structure;the strain D.orixae was identified by Fourior transform infrared analysis self-carrying hydroxyl,carbonyl,amide bond,and other functional groups may contribute to the formation and stabilization of silver nanoparticles.In addition,the silver nanoparticles synthesized in this experiment have good catalytic activity for 4-nitrophenol,methyl orange and methylene blue.Endophytic fungus D.orixae the hypha lysate can be used for green synthesis of silver nanoparticles,and the synthesized silver nanoparticles have potential research value in green catalytic degradation of 4-nitrophenol and organic dyes.

关 键 词：纳米银 生物合成 臭常山 Diaporthe orixae sp.nov. 催化活性 

分 类 号：O643.36[理学—物理化学] TB383.1[理学—化学]