MOFs仿生酶传感器构筑及对肌氨酸的高灵敏检测  

Construction of MOFs Bionic Enzyme Sensor and Highly Sensitive Detection of Sarcosine

作　　者：吴玮轩 杨欣 赵钟兴[1] WU Weixuan;YANG Xin;ZHAO Zhongxing(School of Chemistry and Chemical Engineering,Guangxi University,Nanning 530004,China)

机构地区：[1]广西大学化学化工学院,广西南宁530004

出　　处：《生物化工》2023年第5期37-42,共6页Biological Chemical Engineering

摘　　要：目的:构筑NH2-MIL88B(Fe)/核黄素(NM88B/Rf)作为级联纳米酶用于超灵敏的电化学方法检测肌氨酸(Sar)。方法:选择具有类过氧化物酶活性的NM88B作为载体,通过电沉积与Rf建立完整的双纳米酶体系,Rf可将Sar催化为H2O2,NM88B进一步将生成的H2O2转化为·OH。该体系通过缩短双酶间催化间距,提升Rf的催化Sar能力,同时利用酶级联策略实现信号放大,甲基蓝(MB)捕获·OH出现明显电信号变化,显著提高Sar检测灵敏度。结果:该电化学传感器可在1 nmol/L~10 mmol/L范围内实现Sar的高灵敏度定量检测,检出限为0.43 nmol/L,比其他生物传感器低3个数量级。Objective:Construct NH2-MIL88B(Fe)/riboflavin(NM88B/Rf)as a cascade nanase for ultrasensitive electrochemical detection of sarcosine(Sar).Methods:NM88B with peroxidase-like activity is selected as a carrier to establish a complete double-nanometer enzyme system with Rf by electrodeposition.Rf can catalyze Sar to H2O2,and NM88B can further convert the generated H2O2 to·OH.The system improves the catalytic Sar capability of Rf by shortening the catalytic distance between the two enzymes,and at the same time,the enzyme cascade strategy is used to achieve signal amplification.Methyl blue(MB)captures·OH shows obvious changes in electrical signals,significantly improving the sensitivity of Sar detection.Results:The electrochemical sensor can achieve high sensitivity quantitative Sar detection in the range of 1 nmol/L~10 mmol/L,the detection limit is 0.43 nmol/L,and it’s 3 orders of magnitude lower than other biosensors.

关 键 词：肌氨酸 核黄素 金属有机骨架 生物传感器 纳米酶 

分 类 号：O611.66[理学—无机化学]