miR-21及miR-155与妊娠期糖尿病患者血管内皮功能的相关性  

Correlation between miR⁃21 and miR⁃155 and vascular endothelial function in gestational diabetes mellitus

作　　者：陈丽霞 禤文婷[1] 陈松锦[1] 黄广英[1] 张秀薇[1] CHEN Lixia;XUAN Wenting;CHEN Songjin;HUANG Guangying;ZHANG Xiuwei(Department of Endocrinology,Dongguan Peoples Hospital,Dongguan,Guangdong,China,523000)

机构地区：[1]广东省东莞市人民医院内分泌科,广东东莞523000

出　　处：《分子诊断与治疗杂志》2023年第11期1873-1876,1881,共5页Journal of Molecular Diagnostics and Therapy

基　　金：广东省医学科研基金(C2018053)。

摘　　要：目的 探究miR-21及miR-155与妊娠期糖尿病(GDM)孕妇血管内皮功能的相关性。方法 选取东莞市人民医院2019年9月至2022年8月收治的102例GDM孕妇作为GDM组,另外选取同期46名于本院行体检的健康孕妇为对照组。比较两组孕妇的一般资料及空腹时血糖(FBS)、胰岛素(FINS)、血清miR-21、miR-155水平和肱动脉的血管内皮依赖性舒张变化率(FMD)、平均动脉压(MAP)、血清内皮素-1(ET-1)。将GDM组FMD<6.0%的孕妇分为异常组(n=44),FMD≥6.0%分为正常组(n=58),分析miR-21及miR-155与GDM孕妇血管内皮功能的相关性;采用多因素logistic回归分析GDM孕妇FMD异常的独立危险因素。采用ROC曲线分析miR-21与miR-155对GDM孕妇血管内皮功能的预测价值。结果 GDM组的血清FBS、FINS、ET-1、miR-21、miR-155水平和MAP均高于对照组,FMD低于对照组,差异均有统计学意义(t=11.235、13.460、18.377、35.908、6.992、8.359、4.596,P<0.05);异常组的年龄、BMI、ET-1、MAP、miR-21、miR-155均高于正常组,差异均有统计学意义(t=7.459、3.815、5.240、56.734、5.021、8.527,P<0.05)。Pearson分析结果显示,miR-21、miR-155与GDM孕妇的FMD呈正相关性(P<0.05)。ROC曲线结果显示,miR-21、miR-155及二者联合预测GDM孕妇血管内皮功能的AUC面积分别为0.761、0.883、0.906(P<0.05)。结论 miR-21及miR-155在GDM孕妇血清中呈过度表达状态,且与孕妇的血管内皮功能指标呈正相关性,二指标对于GDM孕妇血管内皮功能具有一定的诊断价值。Objective To investigate the correlation between miR-21 and miR-155 and vascular endothelial function in pregnant women with gestational diabetes mellitus(GDM).Methods A total of 102 pregnant women with GDM admitted to Dongguan Peoples Hospital from September 2019 to August 2022 were selected as the GDM group,and 46 healthy pregnant women who underwent physical examination in our hospital during the same period were selected as the control group.The general data,fasting blood glucose(FBS),insulin(FINS)levels,serum miR-21 and miR-155 levels,vascular endothelium-dependent dilation change rate(FMD),mean arterial pressure(MAP)and serum endothelin-1(ET-1)between the two groups were compared.Pregnant women with FMD<6.0%in GDM group were divided into the abnormal group(n=44)and FMD≥6.0%into the normal group(n=58).The correlation between miR-21 and miR-155 and vascu-lar endothelial function in pregnant women with GDM was analyzed.Multivariate logistic regression was used to analyze the independent risk factors of abnormal FMD in pregnant women with GDM.The ROC curve was used to analyze the predictive value of miR-21 and miR-155 on vascular endothelial function in pregnant wom-en with GDM.Results Serum levels of FBS,FINS,ET-1,miR-21,miR-155 and MAP in the GDM group were higher than those in the control group,but FMD were lower than those in the control group(t=11.235,13.460,18.377,35.908,6.992,8.359,4.596,P<0.05).The age,BMI,ET-1,MAP,miR-21,and miR-155 in the abnormal group were higher than those in the normal group(t=7.459,3.815,5.240,56.734,5.021,8.527,P<0.05).The results of Pearson analysis showed that miR-21 and miR-155 were positively correlated with FMD in pregnant women with GDM(P<0.05).The ROC curve results showed that the AUC area of miR-21,miR-155 and their combined prediction of vascular endothelial function in pregnant women with GDM was 0.761,0.883 and 0.906,respectively(P<0.05).Conclusion miR-21 and miR-155 are overexpressed in the serum of pregnant women with GDM and are positively correlated with the vas

关 键 词：MIR-21 MIR-155 妊娠期糖尿病 血管内皮功能 

分 类 号：R714.256[医药卫生—妇产科学]