藏药西藏凹乳芹及近似种HPLC指纹图谱对比研究  

Comparative study on HPLC fingerprints of Vicatia thibetica de Boiss.and its similar species

作　　者：王艺娱 徐微 齐景梁 高必兴 苟琰 李倩 高驰 王颖 杨蕾 兰志琼[1] WANG Yi-yu;XU Wei;QI Jing-liang;GAO Bi-xing;COU Yan;LI Qian;GAO Chi;WANG Ying;YANG Lei;LAN Zhi-Qiong(Key Laboratory of Standardization of Chinese Herbal Medicine,State Key Laboratory of Traditional Chinese Medicine Resources of Southwestern China,College of Pharmacy,Chengdu University of Traditional Chinese Medicine,Chengdu 611137,China;Sichuan Institute for Drug Control,Key Laboratory of Quality Evaluation of Chinese Patent Medicines,NMPA,Chengdu 611731,China)

机构地区：[1]成都中医药大学药学院中药材标准化教育部重点实验室西南特色中药资源国家重点实验室,成都611137 [2]四川省药品检验研究院国家药品监督管理局中成药质量评价重点实验室,成都611731

出　　处：《药物分析杂志》2023年第11期1925-1934,共10页Chinese Journal of Pharmaceutical Analysis

基　　金：国家药品监督管理局注册司专项特色民族药检验方法示范性研究三期(NIFDC-TCM2021-042-MZY004);中国药品监管科学行动计划第二批重点项目(NMPAJGKX-2023-075);四川省药品监督管理局科技计划项目(2021002)。

摘　　要：目的:建立藏药西藏凹乳芹的HPLC指纹图谱方法,并与其近似种进行对比分析,寻找各品种间成分的共性和差异性。方法:收集主产区具有代表性的26批西藏凹乳芹样品,建立其HPLC指纹图谱方法。采用《中药色谱指纹图谱相似度评价系统》(2012.130723版)对西藏凹乳芹及其近似种(迷果芹、西藏棱子芹、刺果峨参、羽苞藁本、凹乳芹、峨参、东当归、松潘棱子芹)的指纹图谱进行相似度评价分析。同时以共有峰面积量化数据为变量,采用SIMCA软件对临床常用的3个加哇基原药材(西藏凹乳芹、迷果芹、西藏棱子芹)进行主成分分析(PCA)和偏最小二乘法判别分析(PLS-DA)。结果:HPLC指纹图谱共标定了西藏凹乳芹17个共有峰,并指认了L-色氨酸、绿原酸、阿魏酸、异绿原酸A、异绿原酸C、洋川芎内酯I及藁本内酯7个共有峰。通过相似度比较分析,西藏凹乳芹与近似种具有明显差异。PCA及PLS-DA结果显示西藏凹乳芹、迷果芹及西藏棱子芹具有显著差异性,并筛选出6个种间差异的标志物。结论:建立的HPLC指纹图谱结合多元统计学分析可鉴别西藏凹乳芹及其近似种,各品种间成分上存在着较大的差异性,所指认的7个共有峰均为其主要有效成分,为其质量标准的制定打下基础。Objective:To establish the HPLC fingerprint of Vicatia thibetica de Boiss.and to compare its similar species to find out the commonalities and differences of the components among the species.Methods:The HPLC fingerprint method was established by collecting 26 representative batches of Vicatia thibetica de Boiss.samples from the main production area.The chromatography fingerprint similarity evaluation software(2012.130723 version)was used to compare Vicatia thibetica de Boiss.and its similar species,including 14 batches of Sphallerocarpus gracilis(Bess.)K.-Pol.,4 batches of Pleurospermum hookeri var.thomsonii C.B.Clarke,1 batch of Anthriscus.nemorosa(M.Bie.)Spreng.,6 batches of Ligusticum daucoides(Franch.)Franch.,1 batch of Vicatia coniifolia(Wall.)DC.,2 batchs of Anthriscus sylvestris(L.)Hoffm.,1 batch of Angelica acutiloba(Sieb.et Zucc.)Kitagawa,and 5 batchs of Pleurospermum franchetianum Hemsl.Meanwhile,principal component analysis(PCA)and partial least squares discriminant analysis(PLS-DA)were performed on the three clinically commonly used Jiawa species,using SIMCA software with the quantified data of shared peak areas as variables.Results:Seventeen shared peaks were comfirmed,and seven shared peaks of L-tryptophan,chlorogenic acid,ferulic acid,isochlorogenic acid A,isochlorogenic acid C,chuanxiong lactone I and ligustrolactone were identified.The results of similarity evaluation and PCA and PLS-DA showed that there were significant differences among species,and six markers of inter-species differences were screened.Conclusion:The established HPLC fingerprint combined with multivariate statistical analysis can identify Vicatia thibetica de Boiss.and its similar species,and there is a large variability in the composition among the species.The seven shared peaks were identified as the main active ingredients,laying the foundation for the development of its quality standard.

关 键 词：西藏凹乳芹 藏药加哇 迷果芹 西藏棱子芹 种间差异 HPLC指纹图谱 

分 类 号：R917[医药卫生—药物分析学]