基于抓力-运动诱发电位的大鼠脑干锥体束功能损伤程度研究  

Study on Degree of Functional Injury for Brainstem Pyramidal Tract in SD Rats Based on GS-MEP

作　　者：石菁菁 傅倩颖 杨袖菊 蒲山山 王国祥 朱林娜 王迅 尹志勇 刘盛雄 SHI Jingjing;FU Qianying;YANG Xiuju;PU Shanshan;WANG Guoxiang;ZHU Linna;WANG Xun;YIN Zhiyong;LIU Shengxiong(College of Pharmacy and Bioengineering,Chongqing University of Technology,Chongqing 400054,China;Bioengineering College,Chongqing University,Chongqing 400030,China;Equipment Department,Chongqing Seventh People’s Hospital,Chongqing 400054,China;China Automotive Engineering Research Institute Co.,Ltd.,Chongqing 401122,China)

机构地区：[1]重庆理工大学药学与生物工程学院,重庆400054 [2]重庆大学生物工程学院,重庆400030 [3]重庆市第七人民医院设备科,重庆400054 [4]中国汽车工程研究院股份有限公司,重庆401122

出　　处：《医用生物力学》2023年第5期1031-1036,共6页Journal of Medical Biomechanics

基　　金：国家重点研发计划项目(2016YFC0800702-3);重庆理工大学研究生教育高质量发展行动计划(gzlcx20222091)。

摘　　要：目的探讨利用抓力(grasp strength,GS)-运动诱发电位(motor evoked potential,MEP)方法对大鼠脑干锥体束损伤后产生的运动功能障碍进行定量研究。方法雄性SD大鼠30只,分为1.25 m致伤组、2.25m致伤组和正常组,采用经典Marmarou模型制作锥体束部位的创伤性轴索损伤模型,致伤组均于损伤后1、3、5、7、14、28、42 d对大鼠进行肢体GS信号和MEP波幅检测,3组大鼠均于麻醉后相应时间进行对照检测。结果致伤组随损伤程度的增加,MEP波幅和最大GS下降比例明显增大;两个高度下GS与MEP测量值具有明显正相关性。损伤程度较小时,前期MEP比较敏感,后期MEP不敏感;损伤程度较大时,前期与后期MEP变化均较为敏感。结论采用GS-MEP联合评估可以为锥体束损伤后产生的运动功能障碍程度的定量评价提供数据支持。Objective To quantitatively investigate the motor dysfunction after brain stem pyramidal tract injury in rats by grasp strength(GS)-motor evoked potential(MEP).Methods Thirty healthy male SD rats were randomly divided into 1.25 m injury group,2.25 m injury group and normal group.The classical marmarou model was used to make the traumatic axonal injury model of pyramidal tract.In injury group,the limb GS signal and MEP were detected at 1^(st),3^(rd),5^(th),7^(th),14^(th),28^(th) and 42^(nd) day after injury,and the control test was carried out at the corresponding time after anesthesia.Results With the increase in injury degree,the MEP amplitude and maximum GS decreased significantly in injury group.The measured values of GS and MEP under two heights had an obvious positive correlation.When the damage degree was relatively small,MEP was more sensitive at early stage,but MEP was not sensitive at late stage.When the damage degree was relatively large,MEP changes at both early and late stages were more sensitive.Conclusions The combined evaluation of GS-MEP can provide data support for the quantitative evaluation of motor dysfunction after pyramidal tract injury.

关 键 词：交通事故 脑干锥体束 颅脑损伤 抓力 运动诱发电位 

分 类 号：R318.01[医药卫生—生物医学工程]