玉米AGPase小亚基基因的扩张分析  

Evolutionary Analysis of AGPase Small Subunit in Maize

作　　者：鲁佳林 陈娇 李瑞雪 王谢琴 张红 于小蓉 刘汉梅 LU Jia-lin;CHEN Jiao;LI Rui-xue;WANG Xie-qin;ZHANG Hong;YU Xiao-rong;LIU Han-mei(College of Life Science,Sichuan Agricultural University,Ya'an 625000,China)

机构地区：[1]四川农业大学生命科学学院,四川雅安625000

出　　处：《玉米科学》2023年第5期25-33,共9页Journal of Maize Sciences

基　　金：国家自然科学青年基金项目(31601317)。

摘　　要：采用生物信息学方法分析AGPase家族基因的系统发育关系和基因结构特征,基于玉米B73参考基因组(AGPv4)新鉴定玉米AGPase小亚基编码基因,同时根据序列比对结果重新注释基因组中发生扩张的AGPS片段基因。结果表明,新鉴定并重新注释的ZmAGPS2-3、ZmAGPS2-4、ZmAGPS2-5基因包含1个序列高度相似的外显子,由祖先基因ZmAGPS2-2的多个外显子(无内含子)序列拼接形成。其编码蛋白的保守结构域存在不同程度的缺失,且这3个基因在染色体上的位置无共线性关系,也不相邻。因此推测,ZmAGPS2-3、ZmAGPS2-4和ZmAGPS2-5基因起源方式为反转录转座复制,在演化过程中丢失了部分序列后成为功能缺失的假基因。The phylogenetic relationship and gene structure characteristics of AGPase family genes were analyzed by bioinformatics method,and the coding gene of AGPase small subunit in maize was newly identified based on the reference genome of maize B73(AGPv4).At the same time,based on sequence alignment results,the expanded AGPase fragment genes in the genome were re-annotated.The newly identified and re-annotated ZmAGPS2-3,ZmACPS2-4 and ZmAGPS2-5 contain an exon with a highly similar sequence,and are formed by splicing several exons(without introns)of the ancestral gene ZmAGPS2-2.The conserved domains of proteins are deleted in different degrees,and the locations of three genes on chromosomes are not collinear or adjacent.Therefore,it is speculated that the origin of ZmAGPS2-3,ZmAGPS2-4 and ZmAGPS2-5 is reverse transposed duplication,and losing some sequences in the evolution,they become pseudogenes missing the function.

关 键 词：玉米 AGPase小亚基 重注释 反转录转座复制 

分 类 号：S513.035.3[农业科学—作物学]