外源6-苄氨基嘌呤(6-BA)对玉米幼苗抗冷性影响研究  

Effects of Exogenous 6-benzylaminopurine(6-BA)on Cold Resistance of Maize Seedlings

作　　者：杨德光[1] 宫磊 王迎博 何丽 孙慧 任静 郑禹琪 宋纯儒 于庆江 翟婧宜 张倩[1] 谢腾龙[1] 李威[1] YANG De-guang;GONG Lei;WANG Ying-bo;HE Li;SUN Hui;REN Jing;ZHENG Yu-qi;SONG Chun-ru;YU Qing-jiang;ZHAI Jing-yi;ZHANG Qian;XIE Teng-long;LI Wei(College of Agronomy,Northeast Agricultural University,Harbin 150030,China)

机构地区：[1]东北农业大学农学院,哈尔滨150030

出　　处：《玉米科学》2023年第5期74-82,共9页Journal of Maize Sciences

基　　金：黑龙江省自然科学基金联合引导项目(LH2021C024);东北农业大学“青年才俊”项目(20QC02);农业部种子管理局“绿色品种特性鉴定评价专项”(111721301354052342)。

摘　　要：为探究外源6-苄氨基嘌呤(6-BA)对低温胁迫下玉米幼苗生理响应的调节作用,以德美亚3号为试验材料,在人工气候箱中进行盆栽试验。设4个处理,分别为25℃下叶面喷施清水(CK)、25℃下叶面喷施6-BA(6-BA)、5℃下叶面喷施清水(LT)、5℃下叶面喷施6-BA(LT+6-BA)。5℃处理7 d后25℃处理3 d。结果表明,常温(25℃)条件下外源6-BA对玉米幼苗的生长具有一定的促进效果,低温(5℃)胁迫抑制玉米幼苗的生长。在低温条件下,6-BA处理能改善根系生长情况,提高叶绿素含量、净光合速率、气孔导度及最大光化学效率;同时提高抗氧化酶活性,使H_(2)O_(2)和MDA含量降低,缓解低温胁迫对玉米幼苗生长的抑制程度。综上所述,外源6-BA在低温胁迫条件下能通过提高玉米幼苗的光合能力和抗氧化酶活性,降低膜脂过氧化物含量而提高玉米幼苗对低温的抵抗能力。In order to investigate the regulation of exogenous 6-benzaminopurine(6-BA)on physiological response of maize seedlings under low temperature stress,we used"Demeiya 3"maize variety as experimental material and conduct pot experiments in an artificial climate chamber.Four treatments were set up,foliar spraying of water at 25℃(CK),foliar spraying of 6-BA at 25℃(6-BA),foliar spraying of water at 5℃(LT),foliar spraying of 6-BA at 5℃(LT+6-BA).The maize seedlings were treated at 5℃for 7 days and 25℃for 3 days.The results showed that,6-BA had certain stimulative effect on the growth of maize seedlings under normal temperature(25℃).while low temperature(5℃)stress inhibited the growth of maize seedlings.6-BA treatment under low temperature stress led to a increase in root growth,chlorophyll content,net photosynthetic rate,stomatal conductance and maximum photochemical efficiency.At the same time,the activity of antioxidant enzymes was increased,the contents of H_(2)O_(2) and MDA were decreased,and the inhibition degree of low temperature stress on the growth of maize seedlings was alleviated.In conclusion,exogenous 6-BA can improve the resistance of maize seedling to low temperature by improving photosynthetic capacity,antioxidant enzyme activity and reducing the content of membrane lipid peroxides of maize seedlings under low temperature stress.

关 键 词：玉米 6-苄氨基嘌呤 低温胁迫 光合特性 抗氧化系统 

分 类 号：S513.01[农业科学—作物学]