广西山羊感染性肺炎主要病原菌的分离鉴定及耐药性分析  

Isolation,identification and drug resistance analysis of main pathogens of infectious pneumonia in goats in Guangxi Province

作　　者：吴翠兰 陶立 李军 李常挺 彭昊 冯世文 禤雄标 兰美益 钟舒红 马春霞 贺会利 WU Cuilan;TAO Li;LI Jun;LI Changting;PENG Hao;FENG Shiwen;XUAN Xiongbiao;LAN Meiyi;ZHONG Shuhong;MA Chunxia;HE Huili(Guangxi Province Key Laboratory of Veterinary Biotechnology,Guangxi Veterinary Research Institute,Nanning 530001,China;Key Laboratory of China(Guangxi Province)-ASEAN Cross-border Animal Disease Prevention and Control,Ministry of Agriculture and Rural Affairs of China,Nanning 530001,China)

机构地区：[1]广西壮族自治区兽医研究所广西兽医生物技术重点实验室,广西南宁530001 [2]农业农村部中国(广西)-东盟跨境动物疫病防控重点实验室,广西南宁530001

出　　处：《中国兽医学报》2023年第11期2261-2266,共6页Chinese Journal of Veterinary Science

基　　金：广西重点研发计划资助项目(桂科AB16380106);广西兽医生物技术重点实验室自主研究课题(19-50-40-A-04)。

摘　　要：为了解广西山羊感染性肺炎主要病原菌的流行情况及其对抗菌药物的耐药性,试验采用病原分离与PCR检测的方法分别对2017-2021年采自广西的573份羊感染性肺炎病料进行检测,并对主要致病菌进行了药物敏感性检测。结果显示,共分离鉴定出13种422株致病菌,其感染率为75.92%(435/573),最主要的致病菌是绵羊肺炎支原体、大肠杆菌和肺炎克雷伯菌,感染率分别为60.73%(348/573),31.94%(183/573),15.18%(87/573)。在435份病例中,单一感染、双重感染、三重感染、四重感染和五重感染的感染率分别52.41%(228/435),26.21%(114/435),11.03%(48/435),6.21%(27/435),4.14%(18/435)。混合感染样品中除了大肠杆菌+肺炎克雷伯菌的双重感染外,其余混合感染全部检测出绵羊肺炎支原体。药敏试验结果显示,绵羊肺炎支原体对青霉素G、阿莫西林、头孢西丁、链霉素、阿奇霉素、强力霉素耐药,其耐药率为60%~100%;大肠杆菌对林可霉素、青霉素G、阿莫西林耐药,耐药率为60%~100%;肺炎克雷伯菌对青霉素G、阿莫西林、强力霉素、氟苯尼考、林可霉素耐药,耐药率为70%~100%。结果表明,引起广西山羊肺炎的病原种类多样,而绵羊肺炎支原体、大肠杆菌、肺炎克雷伯菌是其主要病原,分离株均出现了多重耐药。To understand the situation of the main pathogenic bacteria of infectious pneumonia in goats in Guangxi and their resistance to antibiotics,573 samples of disease materials from 2017 to 2021 were detected by pathogen isolation,identification and PCR detection methods,respectively,then the drug sensitivity test was carried out for the main pathogenic bacteria.The results showed that 422 strains of 13 species were isolated and identified,with the infection rates of 75.92%(435/573).The Mycoplasma ovipneumoniae,Escherichia coli and Klebsiella pneumoniae were the main pathogen,with the detection rates of 60.7%(348/573),31.94%(183/573),and 15.2%(87/573),respectively.The infection rates of single infection,double infection,triple infection,quadruple infection and quintuple infection among the 435 samples were 52.41%(228/435),26.21%(114/435),11.03%(48/435),6.21%(27/435)and 4.14%(18/435),respectively.Mycoplasma ovipneumoniae was detected in all the mixed infection samples except the double infection of Escherichia coli and Klebsiella pneumoniae.The results of drug sensitivity test showed that Mycoplasma ovipneumoniae was resistant to penicillin G,amoxicillin,cefoxitin,streptomycin,azithromycin and doxycycline,and the resistance rate was 60%to 100%.Escherichia coli was resistant to lincomycin,penicillin G and amoxicillin,and the resistance rate was 60%to 100%.Klebsiella pneumoniae was resistant to penicillin G,amoxicillin,doxycycline,flufenicol and lincomycin,and the resistance rate was 70%to 100%.This study showed that there were various pathogens causing goat pneumonia in Guangxi,and Mycoplasma ovipneumoniae,Escherichia coli and Klebsiella pneumoniae were the main pathogens,and the isolates showed multiple drug resistance.

关 键 词：感染性肺炎 病原菌 绵羊肺炎支原体 耐药性 

分 类 号：S852.61[农业科学—基础兽医学]