应用原代细胞繁殖非洲猪瘟病毒的研究进展  

Progress in the propagation of African swine fever virus using primary cells

作　　者：左媛媛[1,2] 路皓东 巩明霞 郑东霞[1] 王英丽[1] 迟田英[1] 莫绍江[1,4] 徐福朋 徐天刚 戈胜强[1,2] ZUO Yuanyuan;LU Haodong;GONG Mingxia;ZHENG Dongxia;WANG Yingli;CHI Tianying;MO Shaojiang;XU Fupeng;XU Tiangang;GE Shengqiang(China Animal Health and Epidemiology Center,Qingdao,Shandong 266032,China;Key Laboratory of Animal Biosafety Risk Prevention and Control(South),Ministry of Agriculture and Rural Affairs,Qingdao,Shandong 266032,China;Shandong Agriculture University,Tai'an,Shandong 271001,China;Tarim University,Alar,Xinjiang 843300,China;Qingdao Baomaide Biotechnology Co.,LTD.,Qingdao,Shandong 266237,China)

机构地区：[1]中国动物卫生与流行病学中心,山东青岛26603 [2]农业农村部动物生物安全风险预警及防控重点实验室(南方),山东青岛266032 [3]山东农业大学,山东泰安271001 [4]塔里木大学,新疆阿拉尔843300 [5]青岛宝迈得生物科技有限公司,山东青岛266237

出　　处：《中国兽医学报》2023年第11期2377-2382,2388,共7页Chinese Journal of Veterinary Science

基　　金：江苏省重点研发计划(现代农业)重点资助项目(BE2020398);国家重点研发计划资助项目(2022YFD1800500)。

摘　　要：非洲猪瘟(African swine fever,ASF)是影响养猪业发展的最重要疫病之一,其病原体为非洲猪瘟病毒(African swine fever virus,ASFV)。由于该病毒基因组庞大,生物学特性复杂,其增殖、分离通常需要使用猪原代细胞并且只能在经授权的生物安全三级以上实验室进行,阻碍了对该病的基础研究及疫苗研发。对ASFV开展深入的生物学特性研究和疫苗研发,原代细胞仍然是不可或缺的工具。因此,对目前已报道可用于ASFV繁殖的原代细胞种类、优缺点,以及研究应用现状进行综述,目的是为从事相关工作的科研人员提供思路和参考。African swine fever(ASF) is one of the most important diseases affecting the development of pig industry,and its pathogen is African swine fever virus(ASFV).Due to the large genome and complex biological characteristics of the virus,its proliferation and isolation usually require the use of porcine primary cells and can only be carried out in authorized biosafety level 3 or above laboratories,hindering basic research on the disease and vaccine development.Primary cells remain an indispensable tool for further biological characterization and vaccine development of ASFV.In this paper,the types,advantages and disadvantages of primary cells that can be used for ASFV propagation were reviewed,as well as the status quo of research and application,so as to provide ideas and references for researchers engaged in related work.

关 键 词：ASFV 原代细胞 疫苗 

分 类 号：S852.65[农业科学—基础兽医学]