Makinoella parva,a new species of the genus Makinoella(Oocystaceae,Trebouxiophyceae,Chlorophyta)  

作　　者：Xudong LIU Chen SU Jia FENG Junping Lü Qi LIU Fangru NAN Guoxiang LIU Shulian XIE 

机构地区：[1]School of Life Science,Shanxi Key Laboratory for Research and Development of Regional Plants,Shanxi University,Taiyuan 030006,China [2]Key Laboratory of Algal Biology,Institute of Hydrobiology,Chinese Academy of Sciences,Wuhan 430072,China

出　　处：《Journal of Oceanology and Limnology》2023年第6期2391-2402,共12页海洋湖沼学报（英文）

基　　金：Supported by the National Natural Science Foundation of China(Nos.32370219,31900187,32000167);the Shanxi Province Science Foundation for Youths(Nos.201901D211132,201901D211421);the Scientific and Technological Innovation Programs of Higher Education Institutions in Shanxi(Nos.2019L0073,2020L0524);the Key Laboratory of Algal Biology;Institute of Hydrobiology;Chinese Academy of Sciences(No.2018-002);the Shanxi“1331 Project”。

摘　　要：Makinoella Okada is a genus of coccoid green algae belonging to the family Oocystaceae with typical morphological characteristics.Reports to date included only one type species.Since the genus is rarely reported,there is a lack of taxonomic research.In this study,17 strains of this genus were collected from several different places in China,and their taxonomic studies were conducted based on morphological and molecular phylogenetic analysis.The phylogenetic results based on the analysis of ITS,chloroplast genes rbc L and tuf A showed that the genus was divided into two branches.One of the branches comprised newly collected algae.Compensatory base changes(CBCs)and hemi-compensatory base changes(hemi-CBCs)within the secondary structure of the ITS-2 confirmed the branches as two species.Compared with the type species of Makinoella tosaensis,the cell size of this new branch was only about 50%,and included 2-4 colonial cells.Therefore,based on the smaller cell size,simpler colony composition,independent phylogenetic position and CBCs and hemi-CBCs,we suggest that the new clade should be designated as a new taxon of Makinoella,namely Makinoella parva.Among the four molecular markers using in this study,the rbc L and newly introduced ITS are recommended for species separation,which can help further studies to revise the species and generic concepts of the family.

关 键 词：coccoid green algae Makinoella morphology observation Oocystaceae phylogenetic analysis 

分 类 号：Q949[生物学—植物学]